摘要
目的:探讨超声激活血卟啉(HP)对体外培养的人肺癌细胞NCI-446、PLA-801C和PLA-801D的杀伤效应及机制。方法:以HP二盐酸盐为光敏剂,对不同浓度(5、10和20 g.L-1)HP处理的人肺癌细胞NCI-446、PLA-801C和PLA-801D进行超声波声照(实验组),另设细胞对照组不进行HP处理及超声波声照,用MTT法检测细胞活性(A492值),判断HP对肺癌细胞的杀伤作用。选用10 g.L-1HP和NCI-446细胞株进行声动力疗法(SDT),采用流式细胞仪检测HP对细胞周期及凋亡率的影响。结果:人肺癌细胞NCI-446、PLA-801C和PLA-801D各不同浓度HP实验组与对照组比较,细胞活性明显降低,差异有显著性(P<0.05);NCI-446与PLA-801C、PLA-801D细胞活性比较差异有显著性(P<0.05),而PLA-801C与PLA-801D比较差异无显著性(P>0.05)。流式细胞术检测结果显示,SDT组发生了G0/G1期生长停顿,凋亡率为(19.224±6.552)%,明显高于对照组[(0.320±0.360)%](P<0.01)。结论:超声激活HP对体外培养的人肺癌细胞NCI-446、PLA-801C和PLA-801D有明确的杀伤效应,其发生机制是通过诱导细胞凋亡。
Objective To observe the effects of ultrasound combined with hematoporphyrin (HP) on NCI-446, PLA-80IC, PLA-801D of human lung cancer cell lines in vitro. Methods NCI-446, PLA-80IC, PLA-801D of human lung cancer cell lines which were treated with different concentrations (5, 10, 20 g · L^-2) of HP were used as experimental groups to investigate the killing role of ultrasound with HP as photosensitizer, and the cells in control group were not treated with HP and uhrosound. MTT was adopted to measure A492, STD was used in 10 g · L^-1 Hpand NCI-446 cell lines, then the influence of HP in cell cycle and apoptotic rate was detected with flow cytometry. Results There were significant differences of cell activity between various experimental groups and control group (P〈0. 05) and also between NCI-446, PLA-801C and PLA-801D (P〈0.05); while no difference between PLA-801C and PLA-801D. The result of flow cytometry showed that Go/G1 stage arrest was found in SDT group. The apoptotic rate in SDT group (19. 224 %± 6. 552 %) was higher than that in control group (0. 320 % ± 0. 360%) (P〈0.01). Oonclusion Ultrasound combined with HP has definite killing effect on human lung cancer lines NCI-446, PLA-801C and PLA-801D in vitro through inducting apoptosis.
出处
《吉林大学学报(医学版)》
CAS
CSCD
北大核心
2007年第2期335-337,共3页
Journal of Jilin University:Medicine Edition
基金
吉林省科技厅资助课题(20030426)
吉林大学创新基金资助课题(医学704053)
关键词
血卟啉
超声处理
肺癌细胞
hematoporphyrin
sonication
lung cancer cells