摘要
目的:研究稀土杂多化合物(PTW-6)体外抗乙型肝炎病毒(HBV)的活性。方法:MTT法检测PTW-6对Hep G2 2.2.15细胞的毒性,乙型肝炎病毒e(s)抗原诊断试剂盒检测上清液中HBeAg、HBsAg的含量,Southern blotting法检测PTW-6对细胞内HBV DNA复制的抑制作用。荧光定量PCR分析PTW-6对细胞内HBV mRNA和上清液中HBV DNA含量的影响。结果:PTW-6对2.2.15细胞的半数中毒浓度为1590.46 mg.L-1,PTW-6各浓度实验组对HBeAg和HBsAg的抑制率均高于对照组(P<0.05);随着PTW-6浓度的增高,PTW-6对2.2.15细胞内外HBV DNA的抑制率增加,PTW-6对2.2.15细胞外HBV DNA和细胞内HBV mRNA半数抑制浓度分别为51.1和63.6 mg.L-1。结论:PTW-6体外毒性较低,且对HBV复制有较好的抑制作用。
Objective To evaluate the activity of novel polyoxometalate against hepatitis B virus （HBV） in vitro. Methods MTT assay was used to evaluate the growth inhibitory effects of polyoxometalate on 2.2.15 cell lines, Hepatitis B e （s） antigen （HBeAg） and Hepatitis B surface antigen in the culture medium were determined by ELISA. Southern blotting was performed to detect HBV DNA in Hep G2 2.2.15 cells. Real-time quantity PCR was applied to detect the contents of introcellular HBV mRNA and extracellular HBV DNA. Results The median toxicity concentration （TC50） was 1590. 46 mg · L^-1 on 2.2.15 cells. The inhibitory rats of PTW-6 on HBeAg and HBsAg were markedly higher compared with control group （P〈0. 05）. The median inhibitory concentration （IC50） of PTE-6 in extracellular HBV DNA and introcellular HBV mRNA were 51.1 and 63.6 mg · L^-1 , respectively.Conclusion Novel polyoxometalate is a potential compound for treatment chronic HBV infection.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第2期223-226,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(20171010)
