摘要
目的研究伽玛刀(Gamma knife)立体定向放射外科照射大鼠正常脑组织后亚急性期胶原纤维酸性蛋白(GFAP)的表达随时间的变化,探讨星形胶质细胞(AS)的增殖与放射损伤的关系。方法30只成年雌性Wistar大鼠,随机分为6组,每组5只。其中1组为假照射组,其余5组行伽玛刀照射。运用Leksell 23004B型伽玛刀4mm准直器以50Gy照射大鼠右侧尾壳核。不同组别的大鼠分别在照射后1、2、4、8、12周深度麻醉下断头取出脑组织,行免疫组织化学染色观察GFAP的表达。结果照射后4周靶区内GFAP阳性细胞数目开始增多,细胞形态变得不规则。至8周时GFAP阳性细胞进一步增多,胞体变大,胞浆染色较深。照射后12周GFAP阳性细胞数目最多,胞体明显肥大,形态各异,细胞突起粗大不规则,胞浆呈深棕色。结论伽玛刀放射外科以50Gy照射大鼠单侧尾壳核后,照射靶区GFAP阳性细胞数不断增加,细胞形态肥大、形状不规则。
Objective To explore the time-related expression of GFAP in the normal rat brain during the subacute phase after Gamma knife irradiation and the possible relationship between the expression of GFAP and CNS radiation injury. Methods 30 adult female Wistar rats were randomly divided into six groups, five rats in each group. One group served as sham irradiation group while the other five groups underwent Gamma knife irradiation. The right caudate-putamen nucleus was selected as the target of irradiation. A 4 mm collimator was used to transmit 50 Gy dosage to the target region. Rats were killed by groups at 1,2, 4, 8, 12weeks after irradiation. Serial brain sections were processed with the immunohistochemical avidin-biotin complex technique, using anti-GFAP as the primary antibody. Results The number of GFAP positive cells （astrocytes） in the target region 1 or 2 weeks after irradiation was statistically not different from that of the sham group. GFAP positive cells in the target region gradually increase from 4 weeks to 12 weeks after irradiation compared with the sham group. The shape of astrocytes became irregularly over time. Conclusions The expression of GFAP were up-regulated in the caudate-putamen nucleus after 50 Gy Gamma knife radiosurgery and increased over time. Active astrocytosis may play an important role in the cascade of pathophysiological changes in the normal brain after Gamma knife radiosurgery.
出处
《临床神经外科杂志》
CAS
2007年第1期35-38,共4页
Journal of Clinical Neurosurgery
