摘要
目的:探讨强力霉素(Doxycycline,Dox)对大鼠骨髓来源树突状细胞(rat bone marrow-derived dendriticcells,RBM-DCs)表型和功能的影响。方法:无菌分离SD大鼠骨髓细胞,经重组大鼠GM-CSF(recombinated rat GM-CSF,rrGM-CSF)4 ng/ml+重组大鼠IL-4(recombinated rat IL-4,rrIL-4)4 ng/ml+Dox 100 ng/ml,500 ng/ml和1 000ng/ml诱导8天,同时设立rrGM-CSF 4 ng/ml+rrIL-4 4 ng/ml为对照组。于激光共聚焦显微镜下观察Dox对RBM-DCs形态学变化的影响;采用流式细胞仪分析RBM-DCs表面标志的变化;最后用同种异体混合淋巴反应检测RBM-DCs对同种异体T淋巴细胞的刺激能力。结果:激光扫描共聚焦显微镜下观察细胞形态未发现各组与对照组之间有明显差别;流式细胞术分析表明:不同剂量的Dox均可下调未成熟RBM-DCs表面CD11c,OX62,MHC-Ⅱ,CD86和CD80分子的表达;经Dox处理的RBM-DCs刺激T淋巴细胞增殖的能力下降。结论::Dox可能通过调节RBM-DCs表面分子的表达,来调节RBM-DCs的免疫功能。
Objective： To explore the mechanisms of doxycycline （Dox） regulating the immune function of rat bone marrow-derived dendritic cells. Methods： SD rat bone-marrow cells were isolated from rat femur under the asepsis condition. The haemopoietic stem cells were induced by recombinated rat GM-CSF （rrGM-CSF） 4ng/ml, recombinated rat IL-4 （rrIL-4） 4ng/ml combined with Dox 100ng/ml, 500ng/ml or 1000ng/ml respectively for 8 days in vitro. A control was set and induced only by rrGM-CSF 4ng/ml ＋ rrIL-4 4ng/ml simultaneously. The morphology of DCs was monitored by confocal microscope and the cell surface markers on dendritic cells were detected by flow cytometer. The regulatory effects of Dox on the immunostimulatory capacity of the DCs were evaluated by allogeneic mixed leukocytes reaction （MLR） between DCs and T cells. Results： Compared with the control group, DCs＇ morphology hadn＇t changed significantly after being induced by rrGM-CSF ＋ rrIL-4 ＋ Dox; different dose of Dox could down regulate the expression of CDllC, OX62, CD86, CD80 and MHC class Ⅱ on DCs; the Dox-treated DCs inhibted the T cells proliferation significantly in MLR. Conclusion： Dox can down regulate the antigen presenting function of rat bone marrow-derived dendritic cells via decreasing the expression of their surface markers.
出处
《江苏大学学报（医学版）》
CAS
2007年第2期97-101,共5页
Journal of Jiangsu University：Medicine Edition
基金
江苏省科委应用基础项目(BJ99041)
江苏省教委立项项目(98KDJ3004)
江苏省科委社会发展立项项目(SS20115)
国家自然科学基金资助项目(30300169)
国家自然科学基金资助项目(30671984)
