摘要
采用丙酮提取,正己烷萃取,SPE氨基小柱净化,反相HPLC–UV检测法测定草莓中残留四螨嗪。对样品预处理和高效液相色谱条件进行优化。标准曲线的线性范围0.5～100μg/mL。样品加标回收率为92.4%～98.6%,相对标准偏差2.5%～3.9%。采取甲醇–0.5%乙酸水(体积比75∶25)流动相的最佳条件。该法灵敏准确,适用于草莓中农药残留四螨嗪的检测。
A high performance liquid chromatographic using UV （HPLC-UV） detection was developed for determination of clofentezine residues in strawberry. Chromatographic condition and sample pretreatment methods were optimized. The column used was Symmetry Shield^TM RP18 （250 mm ×4.6 mm× 5 μm） with methanol - 0.5% aqueous acetic acid （75 ： 25, V/V） as mobile phase. The average recoveries were between 92.4% and 98.6% and relative standard deviations between 2.5% and 3.9%. The methods is simple, rapid, accurate, sensitive and reproducible.
出处
《现代农药》
CAS
2007年第2期39-40,48,共3页
MODERN AGROCHEMICALS
关键词
四螨嗪
高效液相色谱法
草莓
农药残留
固相萃取
clofentezine
high performance liquid chromatographic
strawberry
pesticide residues
SPE
