摘要
目的:观察糖尿病早期(2周)大鼠背根神经节中胰岛素样生长因子-I(IGF-I)与生长相关蛋白-43(GAP-43)基因的表达变化以及胰岛素对其的影响。方法:按60mg/kg予SD大鼠一次性腹腔注射链脲佐菌素制备胰岛素依赖性糖尿病大鼠模型,然后将造模成功的大鼠随机分成模型组与治疗组,治疗组于造模成功后第2天开始皮下注射胰岛素进行治疗,qd。2周后取各组大鼠背根神经节(DRG),Trizol法提取总RNA,应用RT-PCR法测定IGF-I与GAP-43 mRNA的表达。结果:在糖尿病早期大鼠DRG中,IGF-I,GAP-43 mRNA的表达显著下调,胰岛素能明显上调IGF-I mRNA的表达,对GAP-43 mRNA的表达有上调趋势。结论:在早期糖尿病DRG中IGF-I,GAP-43 mRNA表达下调,参与了糖尿病外周神经病变的发生发展。
Objective： To observe the changes of expression of IGF-I and GAP-43 mRNA in rat dorsal root ganglion of early diabetes and effect of insulin treatment on them. Methods： Insulin-dependent diabetes mellitus model were induced with a single intraperitoneal injection of streptozotocin （STZ, 60mg/kg）. After model was made successfully, the diabetic model rats were divided into two groups （the diabetic model group and the insulin treatment group）. In the second day, the insulin treatment group was injected subcutaneously with protamine zinc insulin once a day. The administration was ceased on the end of the second week, then the dorsal root ganglion of rats in each group were isolated and the total RNA was extracted by using Trizol reagent and the IGF-I and GAP-43 gene expression were detected by the method of RT-PCR. Results： The levels of IGF-I and GAP--43 mRNA were decreased obviously in early diabetic DRG （P〈0.01）, and insulin treatment could up-regulate the gene expression of IGF-I significantly （P〈0.01） and had the tendency of increasing GAP-43 expression. Conclusion： The reductions of IGF-I and GAP-43 mRNA in DRG of early phase of diabetes may contribute to the pathogenesis of diabetic peripheral neuropathy.
出处
《南通大学学报（医学版）》
2007年第2期95-97,100,共4页
Journal of Nantong University(Medical sciences)
