摘要
目的与方法根据不同基因型丙型肝炎病毒（ＨＣＶ）核心区基因序列，合成一条通用引物和四条型特异性引物用于ＲＴ／ＰＣＲ扩增反应，所得不同长度的扩增产物分别对应于ＨＣＶⅠ、Ⅱ、Ⅲ、Ⅳ的基因型，用此方法对采自西藏的６９例藏族和新疆的８１例维吾尔族的丙型肝炎病毒阳性血清进行了分析。结果藏族的６９例中３例为Ⅰ型（３／６９，４．３％），３３例为Ⅱ型（３３／６９，４７．８％），４例为Ⅲ型（４／６９，５．８％），２９例为混合型（２９／６９，４２．１％）；维吾尔族的８１例中８例为Ⅰ型（８／８１，９．９％），１４例为Ⅱ型（１４／８１．１７．３％），３例为Ⅲ型（３／８１，３．７％），５６例为混合型（５６／８１，６９．１％），这同我国内地汉族人群中丙型肝炎病毒基因型分布明显不同。结论 ＨＣＶ基因型的分布有一定的地区特异性。
To analyse the distribution of hepatitis C vinis genotypes in Tibetans and Vighurs.Methods A univeroal primer and four type-specific primers of the core region sequences of different HCV genotypes were used in reverse transcription polymemse chain reaction (RT/PCR) for typing HCV gene. Results The fragment sizes of the PCR products corresponding to genotype Ⅰ、Ⅱ、Ⅲand Ⅳ were 123bp、 211bp、 240bp and 144bp,respectively. The serum samples of 69 HCV infected Tibetans and 81 HCV infected Vighurs were analysed. Among 69 HCV infected Tibetans, three samples (3/69, 4.3%) were type Ⅰ, thirty-three (33/69, 47.8%) were type Ⅱ and four (4/69, 5.8%) were type Ⅲ and twenty-nine (29/69, 42.0%) were multi-genotype. Among 81 HCV infected Wighurs, eight samples (8/81, 9.90%) were typeⅠ, fourteen (14/81, 17.3%) were type Ⅱ, three (3/81, 3.7%) were type Ⅲ and fifty-six (56/81, 69.1%) were multi-genotype. Conclusions The results showed that the distribution of HCV genotypes among Tibetans and Vighurs is probably region-specific, which is different from that of HCV genotypes in inner areas of China.
出处
《中华肝脏病杂志》
CAS
CSCD
1996年第3期154-157,共4页
Chinese Journal of Hepatology
