摘要
对欧李不同外植体对初代培养的影响及增殖阶段不同激素对增殖影响进行了研究。结果表明:欧李外植体以顶芽以下4~8个节位上的腋芽最为合适,萌芽率高,污染和死亡率低,植株生长健壮;增殖阶段不同生长素和细胞分裂素对增殖有不同的影响,适合欧李增殖的生长素为NAA,适合欧李增殖的细胞分裂素为BA,适宜的激素浓度分别为0.04~0.06 mg/L和1.0 mg/L。
In this experiment,studies are given to the effects of explants in initial stage and hormones in regeneration stage on the tissue culture of Prunus hunitis.The result shows that the suitable explant is from 4~8 section axillary bud under the terminal bud,with high germination rate and low contaminated rate and low mortality,and the plants are strong.In regeneration stage,the suitable auxin is NAA,with concentration of 0.04~0.06 mg/L,the suitable cytokine is BA,with concentration of 1.0 mg/L.
出处
《河北林果研究》
2007年第1期62-64,73,共4页
Hebei Journal of Forestry and Orchard Research
基金
北京京海生物工程有限公司资助项目
关键词
欧李
外植体
增殖培养
激素
Prunus humilis
explant
regeneration stage
hormone