摘要
目的:观察长期使用免疫抑制剂致卡氏肺孢子虫肺炎大鼠灌胃苦参合剂后细胞免疫功能的变化情况,探讨中药在器官移植后免疫调节及防治感染中的作用。方法:实验于2005-05/2006-03在吉林医药学院基础部病原学教研室完成。选用成年健康雌性SD大白鼠40只,由哈尔滨医科大学提供(合格证号:02473146)。每鼠皮下注射醋酸可的松25mg/次,2次/周,建立卡氏肺孢子虫肺炎大鼠模型。按随机数字表法分为2组,苦参合剂组和模型组各20只。苦参合剂组用苦参合剂3mL/kg灌胃,由苦参、秦皮、黄柏、麦芽、黄芪及丹参等组成,2次/d。给药6周后麻醉大鼠,采集肺泡灌洗液。①将肺泡灌洗液用冰冻真空抽干法浓缩10倍后,用双抗体夹心法ELISA法测定可溶性白细胞介素2受体水平。②大鼠经摘眼球取血,进行淋巴细胞分类计数。③应用冻干抗体致敏的红细胞花环试剂盒检测CD3+,CD4+亚群。结果:40只大鼠全部进入结果,无脱失。①苦参合剂组大鼠外周血的淋巴细胞分类计数显著高于模型组(5.1±1.3)%,(0.8±0.3)%(P<0.01)。②苦参合剂组大鼠的CD3+,CD4+细胞百分率均显著高于模型组[(46.8±1.9)%,(26.1±2.1)%(P<0.01);(38.4±2.6)%,(17.3±2.0)%(P<0.01)]。③苦参合剂组大鼠可溶性白细胞介素2受体水平低于模型组低(331.7±27.8)U/mL,(474.5±34.4)U/mL(P<0.05)。结论:苦参合剂可以增强卡氏肺孢子虫肺炎模型大鼠的细胞免疫功能,可作为器官移植后长期使用免疫抑制剂致卡氏肺孢子虫肺炎治疗的辅助用药。
AIM： To study the effect of the radix sophorae flavescantis on cellular immunity in rats with Pneumocystis Carinii Pneumonia （PCP） induced by long-term use of immunosuppressant, and explore the action of traditional Chinese medicine for the immunological regulation and infectious prevention after organ transplantation. METHODS： The experiment was conducted at Department of Pathobiology, Jilin Medical College from May 2005 to March 2006. Forty adult healthy female SD rats were selected from Harbin Medical University （Certification： 02473146） and randomly divided into experiment group and control group, with 20 rats in each. The model of PCP was set up by glucocorticoid injection subcutaneously to SD rats （25 mg once, 2 times/week）. The mixture of sophorae flavescentis was given to stomach with tube in experiment group （3 mL/kg, 2 times/day）, and was consisted of radix sophorae flavescantis, ash bark, amur cork-tree, malt, milkvetch root and danshen root. Six weeks later, all the rats were anesthetized and broncholveolar lavage fluids were collected. （1）Alveolar washing fluid was concentrated 10 times and the levels of the soluble interleukin-2 receptor （slL-2R） were examined by double antigen sandwich ELISA. （2） Blood was sampled from rat eyes and the count of lymphocytes in peripheral blood were detected.（3）The percentages of CD3^＋ and CD4^＋ subgroups were assessed with erythrocyte chaplet kit sensitized by antigen. RESULTS： All 40 rats were involved in the result analysis without drop. （1）The count of lymphocytes in peripheral blood in experiment group was significantly higher than that in control group [（5.1 ±1.3）%, （0.8±0.3）%, P 〈 0.01]. （2）The percentages of CD3^＋ and CD4^＋ in experiment group were also significantly higher than those in control group [（46.8±1.9）%, （26.1±2.1）%, P〈 0.01; （38.4±2.6）%, （17.3±2.0）%, P 〈 0.01]. （3）The level of slL-2R in experiment group was lower than that in control group [（331.7±27
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第12期2378-2379,共2页
Journal of Clinical Rehabilitative Tissue Engineering Research
