摘要
目的检测安徽省1型糖尿病患者的抗原肽相关运载蛋白体(transporter associated with antigen processing,TAP)基因位点多态性。方法采用聚合酶反应-限制片断长度多态性技术,检测16名1型糖尿病患者和50名健康对照TAP位点的多态性。TAP1和TAP2基因分别有2和4个多态性位点,其限制性内切酶分别是TAP1等位基因333位点(TAP1-1,Ile-Val)的Sau3Al内切酶,637位点(TAP1-2,Asp-Gly)的AccI酶;TAP2等位基因379位点(TAP2-1,Val-Ile)的BstuI酶;665位点(TAP2-2,Ala-Thr)的MspI酶;565位点(TAP2-3,Thr-Ala)的RsaI酶以及687位点(TAP2-2,Stop-Gln)的BfaI酶。结果病例组和对照组的TAP1等位基因型分别以637 Gly和637Asp为主;TAP2等位基因分别以379Ile和379Val为主,两组间差异均有统计学意义(P<0.01)。其余等位基因型差异均无统计学意义(P>0.05)。结论在1型糖尿病患者与正常对照人群间的某些TAP基因位点有差异,提示TAP基因可能与该病有密切关联。
Objective To understand the polymorphisms of transporter associated with antigen processing （TAP） genes in patients with Type 1 Diabetes mellitus in Anhui. Methods 16 Chinese patients with type 1 diabetes mellitus and 50 health controls were selected and their blood samples were collected for the examination of polymorphism of TAP genes. A polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method was used to discriminate two dimorphic sites of TAP1 gene and four dimorphic sites of TAF2 gene. Amplified products from genomic DNA were digested with restriction endonucleases： Sau3A1 for TAP1 cedon 333（Ile-Val）, AccI for codon 637（Asp-Gly）, and BfaI for cedon 687（Stop-Gin）. The other sites of TAP2 gene were analyzed by MspI for cedon 665（Thr- Ala）. Re,tits The preponderant phenotypes of TAP1 allele were 637Gly in type 1 DM and 637 Asp in control group; but In TAP2 allele, were 379Ile in type I DM and 379Val in control group, respectively. There were four TAP1 alleles and seven TAP2 alleles observed in two groups. The alleles phenotype frequencies of TAP1G, TAP1C and TAP2D were significant differences between two groups （P〈0.01）. Condttqion The results implied that there was a relation between TAP genes and type 1 DM.
出处
《中国慢性病预防与控制》
CAS
2007年第1期28-31,共4页
Chinese Journal of Prevention and Control of Chronic Diseases
