摘要
目的:探讨银杏叶提取物(EGb761)对慢性乙型肝炎患者肝窦微循环障碍的影响.方法:将47例慢性乙型肝炎患者分为EGb761治疗组26例及对照组21例,治疗前后分别进行肝组织活检术,肝组织行HE染色,并各取10例电镜观察治疗前后的肝窦微循环变化.结果:治疗后肝活检证实,与治疗前(炎症计分:15.7±6.3;纤维化计分:11.9±6.2)和对照组治疗后(炎症计分:14.2±6.6;纤维化计分:11.5±5.6)相比,EGb761治疗组肝组织损伤程度及纤维化程度明显改善(炎症计分:10.7±4.8;纤维化计分:8.3±4.2)(P<0.05);与治疗前相比,EGb761治疗组电镜下肝窦内血细胞聚集或微血栓形成(32vs56,P<0.01)、窦腔及Disse隙胶原沉积(23vs38,P<0.05)、窦内皮损伤(41vs59,P<0.05)、毛细血管化(17vs32,P<0.05)等肝脏微循环障碍明显改善;而对照组治疗前后均无明显变化(P>0.05).结论:银杏叶提取物能保护慢性乙肝患者肝窦内皮细胞,抑制肝窦内血细胞聚集及微血栓形成,抑制肝窦及Disse隙内胶原沉积及肝窦毛细血管化,改善肝脏微循环.
AIM： To evaluate the effect of Ginko biloba extract （EGb761） on the microcirculatory dysfunction of hepatic sinusoid in patients with chronic hepatitis B.
METHODS： Forty-seven chronic hepatitis B patients were randomly divided into EGb761 treatment group （n = 26） and control group （n = 21）. All the cases underwent liver biopsy before and after treatment. The pathological changes of liver tissues were detected by HE staining. At the same time, 10 samples were selected from each group, and the changes of hepatic sinusoidal microcirculation were analyzed by electron microscopy before and after treatments.
RESULTS： After treatment, liver cirrhosis and tissue damages were improved significantly （P 〈 0.05） in EGb761 treatment group （inflammation score： 10.7 ± 4.8; fibrosis score： 8.3 ± 4.2） as compared with those in patients before treatment （inflammation score： 15.7 ± 6.3; fibrosis score： 11.9 ± 6.2） and the controls （inflammation score： 14.2 ± 6.6; fibrosis score： 11.5 ± 5.6）. Meanwhile, electron microscopy showed that liver rnicrocirculatory dysfunction was improved obviously in EGb761 group after treatment in comparison with that before treatment, and the congregation of red cells in liver sinus （32 vs 56, P 〈 0.01）, the sedimentation of collagen in hepatic sinusoid and Disse space （23 vs 38, P 〈 0.05）, the damage of hepatic sinusoidal endothelial cells （41 vs 59, P 〈 0.05）, and the hepatic sinusoidal capilarization （17 vs 32, P 〈 0.05） were markedly alleviated. But no difference was found before and after treatment in control group （P 〉 0.05）.
CONCLUSION： EGb761 can improve hepatic microcirculation possibly through protecting hepatic sinusoidal endothelial cells from injury, inhibiting aggregation of blood cells, collagenous proliferation in sinusoid or Disse space and hepatic sinusoidal capilarization.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第7期750-754,共5页
World Chinese Journal of Digestology
基金
山东省科委优秀中青年科学家基金
No.03BS016~~
关键词
银杏叶提取物
慢性乙型肝炎
肝窦
肝脏微循环
Ginko biloba extract
Chronic hepatitis B
Hepatic sinusoid
Hepatic microcirculation
