摘要
目的观察小鼠经8Hz／16Hz，130dB次声作用后脑超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）及丙二醛（MDA）的变化，并同时观察姜黄素的脑保护作用。方法选取72只BALB／C小鼠，将其随机分为空白对照组、单纯次声组及姜黄素＋次声组（包括高、中、低剂量亚组）。姜黄素＋次声组小鼠每日给予不同剂量的姜黄素干预，7d后停止给药；随后将各组小鼠置于8Hz或16Hz，130dB的次声环境中，每天持续2h，7d后处死小鼠，测定其脑组织中SOD、GSH-Px及MDA的变化情况，并进行组间结果对比。结果8Hz次声实验部分：与空白对照组比较，单纯次声组小鼠脑组织SOD及GSH-Px活性下降，MDA含量上升（P〈0．05），次声＋姜黄素中、高剂量组与单纯次声组比较，前者脑组织中GSH-Px及SOD活性上升，MDA含量下降（P〈0．05）。16Hz次声实验部分：与空白对照组比较，单纯次声组小鼠脑组织GSH-Px活性及MDA含量上升（P〈0．05），次声＋姜黄素中、高剂量组与单纯次声组比较，前者脑组织中GSH-Px活性及MDA含量均显著下降（P〈0．05）。结论8Hz／16Hz，130dB次声可导致小鼠脑组织GSH-Px、SOD活性变化及脑组织过氧化损伤，不同频率次声对脑组织抗氧化酶的影响作用不同，姜黄素可通过抗氧化作用缓解因次声诱发的脑损伤。
Objective To explore changes in the levels of superoxide dismutase （SOD） , glutathione peroxidase（GSH-Px） and malondialdehyde （MDA） in the brains of mice after exposure to 8 Hz and 16 Hz infrasound at 130 dB, and effects of Curcumin. Methods BALB/C mice were given various doses of Curcumin daily for 7 d. They were then exposed to 8 Hz or 16 Hz infrasound at 130 dB 2 h per day for 7 d. The mice were then sacrificed and the expression of SOD, GSH-Px and MDA in their brains was measured. Results The 8Hz group showed decreased expression of GSH-Px and SOD,while MDA expression was increased in comparison to a control group. In rats which had been treated with Curcumin, expression of GSH-Px and SOD was increased, and MDA was decreased compared with the untreated mice and the controls. With 16 Hz infrasound, the expression of GSH-Px and MDA was increased in the infrasound group compared with the controls. In the mice treated with Curcumin, the expression of GSH-Px and MDA was lower than in the untreated mice. Conclusion Eight Hz or 16 Hz infrasound at 130 dB may induce changes in GSH-Px and SOD expression and peroxidation in the brains of mice. The effects of antioxidase depend on the frequency. Curcumin can alleviate the damage through antiperoxidation.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2007年第2期104-106,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
全军医学科学技术“十五”计划指令性课题资助项目（011071）
