摘要
为了解H5N1亚型禽流感病毒经自然途径感染SPF鸡后,病毒的致病能力与NS基因的关系,本文主要从病理学角度比较了两株利用反向基因操作技术拯救的病毒RGSGD/1/96和RGSGD/1/2NS的致病能力。虽然只有NS基因不同,但是这两株病毒经鼻腔感染4周龄SPF鸡后表现出完全不同的致病能力,RGSGD/1/96对鸡的致死率为100%,感染鸡只的各组织脏器均可发现严重的病理损伤,该病毒在鸡体内复制能力很强,感染后3d、6d,各组织脏器均可发现大量的病毒抗原;GSGD/1/2NS对鸡的致死率为0,病毒在感染鸡体内只引起肺间质少量淋巴细胞浸润,免疫组织化学检查未发现病毒抗原信号。由于两株病毒只有NS基因不同,说明NS基因决定了H5N1亚型禽流感病毒A/goose/Guangdong/1/96对SPF鸡的致病能力。
In order to study the influence of NS gene on virulence of H5NI influenza virus, two virus strains RGSGD/1/96 and RGSGD/1/2NS, each contains a different NS gene, were rescued from plasmids through reverse genetics. The viruses were used to inoculate nasally SPF chickens to compare their pathogenicity. GSGD/1/96 caused 100 % mortality in the SPF chickens following inoculation, whereas all chicken survived from GSGD/1/2NS infection. Furthermore, GSGD/1/96 spread into all the organs tested and resulted in serious pathological changes. In comparison, no viral antigen was detected in the organs after infected GSGD/1/2NS and only minor pathological changes was found in the lung of chicken. The result showed that NS gene was a determinant factor for pathogenicity of A/goose/Guangdong/1/96 in SPF chicken.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2007年第3期170-172,共3页
Chinese Journal of Preventive Veterinary Medicine
