摘要
目的探讨结直肠癌患者外周血和淋巴结细胞角蛋白20(cytokeratin-20,CK20)mRNA表达和临床病理资料之间的关系,并探讨对预测结直肠癌患者的复发转移、评估预后、提供治疗参考的意义。方法应用反转录聚合酶链式反应(reverse transcriptase polymerase chain reaction,RT-PCR)检测80例结直肠癌患者和20例健康对照者外周血和淋巴结中CK20 mRNA。结果健康对照者外周血细胞CK20 mRNA均阴性,结直肠癌患者外周血CK20阳性率为35%(28/80);TNMⅠ+Ⅱ、Ⅲ、Ⅳ期CK20 mR-NA阳性率分别为17%、38%、75%;高中低分化组外周血CK20 mRNA阳性率分别为30%、35%、40%;72例结直肠癌患者416个淋巴结中常规病检有44例结直肠癌患者156个淋巴结发生转移;16例常规病检阴性的40个淋巴结RT-PCR法检测发现存在微转移。结论结直肠癌患者外周血CK20 mRNA阳性率与患者的临床病理分期、肿瘤浸润深度密切相关;结直肠癌微转移检测对重新评估TNM分期指导临床治疗、预测复发与转移及判断预后有一定的临床价值。
Objective To study the relationship between CK20 mRNA in peripheral blood,lymph nodes with colorectal cancer patients and clinical factors and to explore the significance of predicting recurrences, metastasis, prognosis with CK20 mRNA. Method The CK20 mRNA was detected by RT-PCR in 80 patients with colorectal cancer and 20 normal controls. Results CK20 mRNA was negative in peripheral blood from normal controls, the positive rate of CK20 mRNA in patients was 35%（28/80） ,and 17% in stage Ⅰ ＋ Ⅱ ,38% in stage 11,75% in stage Ⅳ .The positive rate was 30% in hign degree histologiclly,35% in moderate degree,40% in low degree. 156 lymph nodes of 44 patients had metastasis by routing histological examination and 40 routing histologically examed negative lymph nodes of 16 patients had metastasis by RT - PCR. Conclusion The positive rate of CK20 mRNA with patients had a significant correlation with TNM stage and carcinoma invasion degree;there was some values to reevaluate clinical stage and predict recurrences, metastasis, prognosis , even help clinical therapy.
出处
《南华大学学报（医学版）》
2007年第2期230-234,共5页
Journal of Nanhua University(Medical Edition)
