摘要
应用兔肺泡巨噬细胞测试系统，选用细胞半数致死浓度（ＬＣ_（５０））、乳酸脱氢酶活性和细胞培养液中的^（５１）Ｃｒ释放量等指标，研究了ＧＢＷ０４１０４（Ｎｏ．４和ＧＢＷ０４１０５（Ｎｏ．５）两种标准铀矿样粉的细胞毒性，并与国产标准石英（Ｑ，ｓ）进行了比较。结果：Ｑ，ｓ、Ｎｏ．５、Ｎｏ．４的ＬＣ_（５０）依次为０．０７２、０．２０１、０．３４４ｍｇ／ｍ１；综合毒性指数（ＣＴＩ）依次为１．００００、０．６０９３、０．３５６６，为定量评价粉尘样品的细胞毒性提供了初步依据。
Using cultured alveolar macrophages of rabbit.LC_(50)s of cultured cells were calculated,the activity of LDH and the release of ￣(51) Cr from prelabeled rabbit alveolar macrophages were measured.The cytotoxicities of two kinds of standard uranium ore dusts ( No.4 and 5 ) were studied and compared with Chinese standard quartz(0.3). The results showed that the LC_(50)s of Q.s,No. 5 and No.4 were 0.072mg/m1,0.201mg/m1, and 0.344 mg/ml respectively. Composite toxicity indexes were1.0000, 0.6093 and 0.3566. Through this study, primary method was established for quantitative assessment of cytotoxicity of dust samples.
出处
《工业卫生与职业病》
CAS
CSCD
1996年第6期330-333,共4页
Industrial Health and Occupational Diseases
关键词
矽肺
标准铀矿样粉
肺泡巨噬细胞
细胞毒性
Standard uranium ore dust
Alveolar macrophage
Cytotoxicity
￣(51)Cr release
