摘要
目的:探讨血清一氧化氮(NO)、IL-6、IL-10、肿瘤坏死因子(TNF)水平与肝硬化患者预后之间的关系.方法:采用硝酸还原酶法、ELISA法、双抗体夹心酶联免疫分析法分别测定30例健康对照者和56例肝硬化患者治疗前后的血清NO,IL-6,IL-10及TNF水平.结果:肝硬化患者的NO,IL-6,TNF水平显著升高,IL-10水平明显降低.治疗后肝功能减退组的NO (129.21±27.32μmol/L vs 92.18±25.68μmol/L,P<0.05)、IL-6(198.5±23.2 mg/L vs 147.0±19.1 mg/L,P<0.05)、TNF(179.2±23.5 pg/dL vs 121.4±17.5 pg/ dL,P<0.05)水平较治疗前有显著升高,IL-10水平则明显下降(33.4±7.2 mg/L vs 51.6±18.5 mg/L,P<0.05),而治疗后肝功能恢复组NO(58.63±12.25μmol/L vs 94.36±23.45μmol/L,P<0.05)、IL-6(90.3±12.7 mg/ L vs 148.5±15.8 mg/L,P<0.05)、TNF(78.2±14.3 pg/dL vs 124.9±20.1 pg/dL,P<0.05)水平较治疗前降低(P<0.05),IL-10水平则明显升高(89.3±18.9 mg/L vs 48.8±9.5 mg/L,P<0.05).结论:NO,IL-6,IL-10,TNF与肝硬化患者预后有关.
AIM： To study the roles of serum nitric oxide （NO）, interleukin-6 （IL-6）, interleukin-10 （IL-10） and tumor necrosis factor （TNF） in the prognosis of patients with liver cirrhosis.
METHODS： Nitrate reductase assay and doubleantibody sandwich enzyme-linked immunosorbent assay were used to examine the serum levels of NO, IL-6, IL-10 and TNF in 56 patients with liver cirrhosis before and after treatment, and 30 healthy individuals served as controls.
RESULTS： High levels of NO, IL-6, and TNF and low level of IL-10 were observed in patients with cirrhosis. After treatment, the serum levels of NO （129.21 ± 27.32 μmol/L vs 92.18 ± 25.68 μmol/L, P 〈 0.05）, IL-6 （198.5 ± 23.2 mg/L vs 147.0 ± 19.1 mg/L, P 〈 0.05）, and TNF （179.2 ± 23.5 pg/dL vs 121.4 ± 17.5 pg/dL, P 〈 0.05） were significantly elevated while the level of IL-10 （33.4 ± 7.2 mg/L vs 51.6 ± 18.5 mg/L, P 〈 0.05） was markedly decreased in patients with aggravated hepatic function as compared with those before therapy. However, the levels of NO （58.63 ± 12.25 μmol/L vs 94.36 ± 23.45 μmol/L, P 〈 0.05）, IL-6 （90.3 ± 12.7 mg/L vs 148.5 ± 15.8 mg/L, P 〈 0.05） and TNF （78.2 ± 14.3 pg/dL vs 124.9 ± 20.1 pg/dL, P 〈 0.05） were decreased while the level of IL-10 （89.3 ± 18.9 mg/L vs 48.8 ± 9.5 mg/L, P 〈 0.05） was elevated in patients with improved hepatic function after treatment in comparison with those before treatment.
CONCLUSION： The serum levels of NO, IL-6, IL-10 and TNF are correlated with the prognosis of patients with liver cirrhosis.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第6期659-662,共4页
World Chinese Journal of Digestology
关键词
肝硬化
一氧化氮
白细胞介素
肿瘤坏死
因子
预后
Liver cirrhosis
Nitric Oxide
Interleukin-6
Interleukin-10
Tumor necrosis factor
Prognosis
