摘要
In order to investigate the angiogenic effect of intercellular adhesion molecule-1 （ICAM-1）, two parts of experiment were performed. Chick embryo chorioallantoic membrane （CAM） assay was used for in vivo angiogenic research. The chick embryos were divided into 4 groups： ICAM-1 group （divided into 3 subgroups, Ⅰ, Ⅱ and Ⅲ） for screening the angiogenic effect of ICAM-1 by adding different concentrations of ICAM-1 （0.1, 0.2 and 0.3 μg/μL） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup; Anti-ICAM-1 group A （divided into 2 subgroups, Ⅰ and Ⅱ） by adding different concentrations of Anti-ICAM-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup to evaluate the effect of ICAM-1 on the survival of microvessels through observing whether Anti-ICAM-1 could induce involution of the microvessels on CAMs; Anti-ICAM-1 group B （divided into 2 subgroups, Ⅰ and Ⅱ ） by adding different concentrations of Anti-ICAM-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 6 after incubation for every subgroup to evaluate whether ICAM-1 involved in embryonic angiogenesis through observing the growth of microvessels on CAMs; Control group： ICAM-1 or Anti-ICAM-1 was substituted by PBS 5 μL on the day 10 or day 6 after incubation. Three days later, the CAMs were photographed in vivo, excised, sectioned and the number of microvessels was counted. In ICAM-1 group, there was increased number of microvessels arranged radially with ＂spoked-wheel＂ pattern around the gelatin sponges. The new microvessels growing perpendicularly to gelatin sponges were observed. The number of the microvessels growing in the CAM mesenchymes around the sponges in 3 subgroups was higher than that in control group （P〈0.01）, however, there was no significant difference among the 3 subgroups （P〉0.05）. In anti-ICAM-1 group A, the radially arranged microvessels were very unclear around the sponges contrast to that
In order to investigate the angiogenic effect of intercellular adhesion molecule-1 （ICAM-1）, two parts of experiment were performed. Chick embryo chorioallantoic membrane （CAM） assay was used for in vivo angiogenic research. The chick embryos were divided into 4 groups： ICAM-1 group （divided into 3 subgroups, Ⅰ, Ⅱ and Ⅲ） for screening the angiogenic effect of ICAM-1 by adding different concentrations of ICAM-1 （0.1, 0.2 and 0.3 μg/μL） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup; Anti-ICAM-1 group A （divided into 2 subgroups, Ⅰ and Ⅱ） by adding different concentrations of Anti-ICAM-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup to evaluate the effect of ICAM-1 on the survival of microvessels through observing whether Anti-ICAM-1 could induce involution of the microvessels on CAMs; Anti-ICAM-1 group B （divided into 2 subgroups, Ⅰ and Ⅱ ） by adding different concentrations of Anti-ICAM-1 （1：100, 1：50） 5 μL into the chick embryo CAMs on the day 6 after incubation for every subgroup to evaluate whether ICAM-1 involved in embryonic angiogenesis through observing the growth of microvessels on CAMs; Control group： ICAM-1 or Anti-ICAM-1 was substituted by PBS 5 μL on the day 10 or day 6 after incubation. Three days later, the CAMs were photographed in vivo, excised, sectioned and the number of microvessels was counted. In ICAM-1 group, there was increased number of microvessels arranged radially with ＂spoked-wheel＂ pattern around the gelatin sponges. The new microvessels growing perpendicularly to gelatin sponges were observed. The number of the microvessels growing in the CAM mesenchymes around the sponges in 3 subgroups was higher than that in control group （P〈0.01）, however, there was no significant difference among the 3 subgroups （P〉0.05）. In anti-ICAM-1 group A, the radially arranged microvessels were very unclear around the sponges contrast to that
基金
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30271345).
