摘要
采用逆转录ＰＣＲ技术自人胎肝细胞中克隆出人全长１０５９ｂｐ的β２ＧＰ１ｃＤＮＡ，经限制性内切酶图谱分析初步证实后，定向插入表达性载体ｐＧＥＸ－２Ｔ，并筛选出带有插入片段的阳性克隆，为研究抗磷脂抗体所针对的抗原及表位奠定了基础。
By using the reversed transcription-PCR technique, a full length β2GP1 cDNA with 1 095 bp was successfully cloned from human neofetal liver cells. Being confirmed by restriction fragment length polymorphism(RGLP) technique, the fragment was directionally inserted to the carrier PGEX-2T. Positive clone containing the inserted fragment were then selected and identified. The study thus provided solid foundation for further study on antiphospholipid antibody-directed antigens or epitopes.
出处
《上海免疫学杂志》
CSCD
北大核心
1996年第6期325-328,共4页
Shanghai Journal of Immunology
基金
卫生部科研基金资助
上海市免疫学研究所科研基金
