摘要
以虎杖带腋芽的茎段为试验材料,研究虎杖组织培养与快速繁殖的适宜条件。试验结果表明,虎杖在MS+6—BA 2.0 mg/L+IBA 0.2 mg/L+3%蔗糖+0.7%琼脂培养基上增殖效果最好,培养30 d,增殖倍数达到3.4以上;在1/2MS+IBA 0.5 mg/L+3%蔗糖+0.7%琼脂培养基上生根效果最好,生根率100%,平均根长2.0 cm,平均苗高3.5 cm,以圃地表土为基质,在温室大棚内间歇喷雾的移栽成活率达96.25%。
The tissue culture and suitable the condition of fast reproduction of Polygonum cuspidatum sieb et zucc were studied in the paper based on the experimental material of the axillary buds of it. The test result indicated that ： The best multiplies effects was using MS ＋ 6 - BA 2.0 mg/L ＋ IBA 0.2 mg/L ＋ 3 % sucrose ＋ 0.7 % agar as the culture medium, raises 30 d, the multiplication multiple achieved above 3.4. The best takes roots effects was using 1/2MS ＋ IBA 0.5 mg/L ＋ 3 % sucrose ＋ 0.7 % agar culture medium, takes root to lead 100 %, average root long 2.0 cm and average seedling height 3.5 cm. Taking the garden surface soil as the matrix, the intermittent atomization transplants the survival rate in the greenhouse awning to reach 96.25 %.
出处
《湖南林业科技》
2007年第1期10-12,共3页
Hunan Forestry Science & Technology
基金
湖南省林业厅项目
关键词
虎杖
组织培养
增殖倍数
移栽
成活率
Polygonum cuspidatum sieb et zucc
the tissue culture
multiplication
transplants
survival rate
