摘要
本文采用ＺｏｒｂａｘＣ８色谱柱，甲醇－０．２％冰醋酸（６２：３８）为流动相，２２９ｎｍ为检测波长，甲苯磺丁脲为内标，乙酸乙酯萃取血浆药物，建立了测定血浆中格列齐特浓度的ＨＰＬＣ法。该法简便、灵敏、快速、准确，格列齐特在０．２５～８．０μ／ｍｌ的浓度范围内线性关系良好（ｒ＝０．９９９１），检测限为０．１５μｇ／ｍｌ（Ｓ／Ｎ＝３：ｌ），平均回收率为９７．６０％，不同浓度水平测定结果的日内和日间精密度（ＲＳＤ）均小于１０．０％。
This paper reports a RP-HPLC method using tolbutamide as internal standard (IS) for the determination of gliclazide (GZ).The LA-3A HPLC instrument used a Zorbax-C8 column (25cm×4. 6mmm 10μm), the mobile phase consisted of methanol-0. 2% acetic acid (62:38) and the flow rate was 0.9ml/min. The UV detection wavelength was 229nm, column temperature was 30℃ and the drug in plasma was extracted by ethyl acetate. This method is simple, sensitive and rapid. Within 0. 25～8.0μg/ml, GZ had a good linearity (r=0. 999l). The meanrecovery of GZ was 97. 60%, the detection limit was 0.15μ/ml (S/N=3 : l) . The withinday precision and day-to-day precision RSD were less than 10. 0% at three drug levels. This method has been used to investigate pharmacokinetics and bioavailability of GZ in healthy human volunteers.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1996年第3期157-160,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
格列齐特
血浆
高效液相色谱法
药代动力学
gliclazide, human plasma, HPLC. pharmacokinetics. bioavailability
