摘要
提出了一个新的利用延迟荧光强度来表征植物光合速率的系统。根据延迟荧光光谱特征,选用硅雪崩光电二极管(Si-APD),使其运行在盖革(Geiger)模式下作为延迟荧光单光子探测器,采用有源抑制和门控模式(AQGC)进行驱动,对温度、偏压、鉴别电平等参数进行了优化。为了验证系统性能,以大豆(Zao Shu No.18)叶片为模型样品进行了延迟荧光衰减动力学和本底的测量以及延迟荧光强度与最大净光合速率的对比测量,实验结果表明延迟荧光的信噪比达到1000:1,延迟荧光强度与最大净光合速率的线性相关度达到0.984。该系统具有准确和受外界环境影响小的优点。
A novel photosynthesis rate system was proposed based on plant light-induced Delayed Fluorescence （DF）. According to the characteristics of DF spectrum, single photon detection system was adopted based on Si Avalanche Photo Diode （APD）, which worked at Geiger mode and Active Quenching and Gate Control （AQGC）. Optimal design of the parameters such as temperature, bias voltage and discriminator level was accomplished. To demonstrate the ability of the system, took the leaves of soybean （Zao Shu No. 18） as the model sample. The DF decayed kinetics, background and contrast measurement between maximum DF intensity and corresponding Pn were accomplished. The results showed that SNR of DF was 1000：1. Correlation between DF intensity and maximum Pn was 0.984. The developed system has the advantages of high precision and less influence of the environment.
出处
《光电工程》
EI
CAS
CSCD
北大核心
2007年第3期118-122,共5页
Opto-Electronic Engineering
基金
国家自然科学基金面上项目(60378043
30470494)
广东省自然科学基金项目(015012
04010394)
广东省科技计划项目资助(2004B10401011)
关键词
延迟荧光
光合速率检测系统
单光子
相关性
Delayed fluorescence
Photosynthesis rate detection system
Single photon
Correlation
