摘要
采用汉坦病毒Ｍ片段特异性核苷酸分型引物，对Ｒ３６等７株汉坦病毒进行反转录和聚合酶链式反应扩增鉴定，结果Ｒ３６株仅能被Ⅰ型分型引物扩增出特异性片段，而不能被Ⅱ型引物扩增；ＰＣＲ产物的序列分析结果表明，Ｒ３６与ＨＴＮ型病毒７６－１１８株的同源性为７８．４％，与ＳＥＯ型病毒Ｒ２２株的同源性为６８．１％。文中对Ｒ３６与汉坦病毒其它毒株的核苷酸同源性进行了配对比较。该项研究为从核苷酸水平对Ｒ３６株的分型，提供了科学依据。
R36 and other 6 strains of Hantavirus were indentified by reverse transcription and polymerase chain reaction (PCR ) ,using the type-specific oligonucleotide primers based on the sequences of 76-118 and R22 M genomic segments. The results showed that the R36 could only be amplified by type I specific primers , not by type I specific primers. The sequence analysis of PCR products revealed that the sequence similarity of R36 between 76-118 and R22 were 78. 4% and 68. 1% , respectively. In addition , a pairwise comparison of the nucleotide homologies between R 36 and other strains of Hantavirus were made. Thus ,a scientific evidence for the typing of R36 at the nucleotide levcl was provided by this study.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第3期203-206,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
军队医药卫生科研基金
关键词
汉坦病毒
基因分型
R36株
Hemorrhagic fever with renal syndrome (HFRS)
Hantavirus
Genomic typing
