摘要
目的:探讨培哚普利联合厄贝沙坦对扩张型心肌病(DCM)大鼠心肌血管紧张素Ⅱ1型受体(AT_1 Rs)和核纤层蛋白A (lamin A)mRNA表达的调节。方法:腹腔注射阿霉素建立SD大鼠DCM模型。13周后大鼠分为4组:A组为正常大鼠,B组为DCM大鼠,均不予药物干预;C、D组均为DCM大鼠,C组予以培哚普利,D组予以培哚普利联合厄贝沙坦。超声心动图检测大鼠左室射血分数(LVEF);RT—PCR方法检测AT_1Rs、lamin A表达。结果:(1)C、D组干预后LVEF明显升高(P＜0．01),D组LVEF高于C组(P＜0．05)。(2)与A组比较,B组AT_1Rs表达下调(P＜0．05);与B组比较,C、D组AT_1 Rs表达上调(P＜0．05),D组AT_1Rs表达上调更明显。(3)与A组比较,B组lamin A表达下调(P＜0．05);D组lamin A表达水平高于B组(P＜0．05)。结论:DCM的发展伴随心肌AT_1Rs及lamin A表达下调。培哚普利联合厄贝沙坦使AT_1Rs表达明显上调及lamin A表达上调,有利于DCM心力衰竭的治疗。
Objective：To determine the mRNA expression of Angiotensin Ⅱ type 1 receptors （ AT1Rs ） and lamin A regulated by combination therapy with perindopril and irbesartan in the myocardium of a rat model of dilated cardiomyopathy （ DCM ）. Methods：Sprague-Dawley rats were administered adriamyein intraperitoneally to develop DCM. 13 weeks later, the survivors were divided into 4 groups, group A contained normal rats, and group B contained rats with DCM. Both these groups were not given drug treatment. Group C and groups D contained rats with DCM, however, group C was administered perindopril and group D was administered perindopril and irbesartan. Left ventricular ejection fraction （ LVEF ） was measured by echocardiogram and the expression of＂ AT1Rs and lamin A was detected by RT-PCR. Results： （1） LVEF in both goup C and group D as much higher after intervention compared with that before intervention （ P〈0.01 ）, and group D LVEF was higher than that of group C （P〈0.05）. （ 2 ） Compared with group A, group B expression levels of AT1Rs were down-regulated （P〈0.05）. Compared with B group B, both group C and group D expression levels of AT1Rs were upregulated （ P〈0. 05 ）, and group D expression levels of AT1Rs were more obviously upregulated. （ 3 ） Compared with group A, group B expression levels of lamin A were down-regulated （ P〈0.05 ）. Group D expression levels of lamin A were higher than those of group B（ P〈0.05 ）. Conclusion：The development of DCM is hccompanied by down regulation of the expression of AT1Rs and lamin A. Combination therapy with perindopril and irbesartan can obviously up-regulate the expression of AT1Rs and up-regulate the expression of lamin A, which may contribute to the better therapeutic effects in the heart failure of DCM.
出处
《中国临床医学》
北大核心
2007年第1期46-49,共4页
Chinese Journal of Clinical Medicine
基金
湖南省教育厅"十五"重点学科建设项目[湘教发(2005)100]
