摘要
从石油污染的污泥中分离驯化出10株细菌(SB01～SB10),利用生物摇床实验对其降解苯并芘(BaP)的效能进行试验,研究了有(或无)共基质(葡萄糖Glu,或菲PHE)对细菌降解BaP的影响,并采用ANOVA和Tukey多重比较进行分析。结果表明(,1)当以BaP为惟一碳源和能源且BaP初始浓度为50mg·L-1时(MS1),SB01的降解率最高,5d可降解31.0%;以Glu为共代谢基质时(MS2),SB09的降解率最高,可达36.9%;以PHE为共代谢基质时(MS3),SB01对BaP的降解率为46.0%。(2)Glu对SB01、SB02、SB03、SB07、SB10降解BaP有抑制作用,对SB01抑制作用最明显,使SB01的降解率降低了13.1%,Glu对SB05,SB08降解率无明显促进或抑制作用。(3)PHE对细菌降解BaP均表现出促进作用,对SB01的促进作用最明显,使其降解率提高15.0%。(4)Glu对SB09的促进作用大于PHE的促进作用,而对SB06,PHE的促进作用大于Glu。
Autochthonous bacteria strains （SB01-SB10） were isolated from a contaminated soil and studied for their potential to degrade ben- zo[a] pyrene, a five-ring polycyclic aromatic hydrocarbon（PAH）, as their only soure of carbon and energy （MS1） or using glucose （MS2） or phenanthrene （MS3） as their primary nutrient co-substrates. One-way ANOVA and Tukey multiple comparisons were used in analyzing the degradation rate of BaP by the ten bacteria strains in different liquid mineral basal medium. The results showed that the biodegradation rate of BaP by different bacteria strains in different medium had significant difference. （1） When the initial concentration of BaP was 50 mg·L^-1, 31.0% of BaP could be degraded by SB01 for 5 days in SM1, which was the highest in the ten different bacteria. In SM2, SB09 had the highest degradation rate for BaP, 36.9% for 5 days. However, 46.0% of BaP were removed by SB01 in SM3. （2） The degradation of BaP for SB01, SB02, SB03, SB07, and SB10 were inhibitioed by glucose, but no significant difference for that of SB05 and SB08. Biodegradation rate was decreased 13.1% for SB01using glucose as the primary nutrient co-substrate compared with in SM1. （3） The biodegradation rate of BaP by these ten bacteria strains were enhanced as phenanthrene as their primary nutrient co-metabolism. （4） For SB09, the biodegradation rate significantly increased by 19.3%using glucose as the primary nutrient co-substrate, more than that by glucose. But for SB06, the role of phenanthrene was higher than the glucose for increasing biosdegradation rate.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第1期290-294,共5页
Journal of Agro-Environment Science
基金
国家自然科学基金资助项目(20337010)
国家重点基础研究发展计划973项目(2004CB418506)
中俄自然资源与生态环境联合研究中心
关键词
PAHS
细菌
生物降解
苯并芘
葡萄糖
菲
共基质
PAHs
bacteria strain
biodegradation
benzo[a]pyrene
glucose
phenanthrene
co-substrate
