摘要
目的探讨抗氧化剂N-乙酰半胱氨酸(NAC)对高血压大鼠心脏重构的影响及其作用机理。方法结扎SD大鼠腹主动脉造成高血压模型,以NAC(75mg/d)干预4周,测大鼠超声心动图、颈动脉血压、左室质量,观察心脏组织谷胱甘肽及基质金属蛋白酶2、9的分布,并观察心脏组织胶原含量的变化。结果与手术组相比,试验组大鼠收缩压明显降低[(161±6)vs(198±10)mmHg,P<0.05],左室收缩功能明显改善[EF(77.2±6.4)%vs(90.6±2.7)%,P<0.05],谷胱甘肽在心肌组织中的含量显著增加,而左室质量及基质金属蛋白酶在心肌组织中的分布均有显著降低,心肌胶原含量明显减少。结论NAC通过增加心肌组织还原型谷胱甘肽含量改善心肌组织氧化还原状态,从而减缓高血压大鼠心脏的进一步重构。
Objective To study the effects of N-acetylcysteine （NAC）, an antioxidant, treatment on cardiac remodeling in hypertensive rat, and its mechanisms. Methods Hypertension model was established by coarctation of abdominal aorta. Sixty-five male SD rats were sacrified at 4 or 8th weeks separately after operation with sham operation groups as control. NAC （75 mg/d） was added to the drinking water for 4 weeks starting from 4 weeks after surgery. Blood pressure（BP） was measured by tail-cuff plethysmography and carotid artery catheterization. Echocardiography, cardiac glutathione, matrix metalloproteinase activities, histological analysis and were determined. Results Four weeks after operation, hypertension and decreased LV shortening fraction were shown, which were accentuated further more at 8th weeks. Meanwhile, cardiac glutathione content was decreased significantly. NAC reduced BP （161±6 vs 198±10 mm Hg, P〈0. 05） and LV remodeling in associated with increases in glutathione in myocardium and decrease in matrix metalloproteinase 2 and 9 and collagen deposition. Conclusion An anti-hypertensive and anti-cardiac remodeling effects on hypertensive rats by NAC was shown which may be related to the increases in cardiac glutathione and inhibition the activities of MMP-2 and MMP-6.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2007年第2期137-140,共4页
Chinese Journal of Hypertension
