摘要
目的:研究选择性血管紧张素Ⅱ受体拮抗剂(缬沙坦)对STZ诱导的糖尿病大鼠血管组织单核细胞趋化蛋白-(1MCP-1)表达的影响。方法:将SD大鼠随机分成三组:正常对照组(NC组)、糖尿病组(DM组)、糖尿病+缬沙坦干预组(DV组)。观察各组大鼠的血糖、体重、Hb1Ac,于8周时用RT-PCR和Westernblot的方法检测链脲佐菌素(STZ)诱导的糖尿病大鼠血管组织中MCP-1的表达的变化。结果:DM和DV组大鼠体重较NC组明显降低(均P<0.01),DM和DV组间糖化血红蛋白(HbA1c)及血糖无显著性差异(P>0.05);DM和DV组大鼠血管组织中MCP-1mRNA的表达明显高于NC组(均P<0.01),而DV组明显低于DM组(P<0.01);DM和DV组大鼠血管组织中MCP-1蛋白的表达明显高于NC组(均P<0.01),而DV组明显低于DM组(P<0.01)。结论:糖尿病SD大鼠血管组织单核细胞趋化蛋白-1(MCP-1)表达明显增加,缬沙坦通过降低血管组织炎症因子MCP-1表达,对糖尿病大血管并发症起防治作用。
Objective：To investigate the effects of Valsartan on the expression of monocyte chemoattractant protein-1 （MCP-1 ） in the arteria of STZ-induced diabetic rats. Methods：SD rats wererandomly divided into NC group,DM group and DV group. Blood glucose（BG）, body weight（BW）, and HbAlc were observed, the expression of MCP-1 in the arteria of diabetic rats was detected by RT-PCR and Western blot at the 8 th week. Results：The BW of diabetic rats in DM and DV groups decreased significantly compared with NC group （P 〈 0.01）, but no notable difference was found in HbAlc and BG between DM and DV group （P 〉 0.05）. The expression of MCP-1 mRNA in DM and DV groups was much higher than that in NC group （P 〈 0.01 ）, but it was much lower in DV group than that in DM group （P 〈 0.01 ）. The expression of MCP-1 protein in DM and DV groups was much higher than that in NC group （P 〈 0.01 ）, and it was much lower in DV group than that in DM group （P 〈 0.01 ）. Conclusion：The expression of MCP-1 is much higher in the arteria of diabetic rats. Valsartan could obviously reduce the expression of MCP-1 in the arteria of diabetic rats and ameliorate the inflammation in the arteria.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期145-149,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅科研基金项目(H200320)
