摘要
目的:观察人类巨细胞病毒(human cytomegalovirus,HCMV)作用于血管内皮细胞(vein endothelialcells,ECV)对E选择素时序性的表达,探讨HCMV感染致动脉粥样硬化(atherosclerosis,AS)发生和发展的可能机制。方法:用HCMV感染ECV,采用RT-PCR方法检测不同感染时段细胞E选择素mRNA及蛋白的表达。结果:HCMV感染ECV后,感染0 h时E选择素mRNA有基础水平的表达,4 h升高并达高峰,8 h时开始回落,12 h回落显著,24 h回落更明显,与0 h比较,差异均有统计学意义(P<0.01),48 h接近0 h表达水平(P>0.05)。结论:HCMV感染ECV后能够促进E选择素表达,并且呈时间依赖性。HCMV有可能通过上调E选择素的表达,介导ECV的炎症反应,促进AS的发生、发展。
Objective： By investigating sequential changes of E-selectin in human umbilical vein endothelial cells （HUVEC） by human cytomegalovirus （HCMV） infection, to study the potential mechanism of atheroscleresis（AS） induced by cytomegalovirus infection. Methods： HUVEC was cultured and then infected by HCMV. Expression of E-selectin mRNA was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results： Four hours after cytomegalovirus infection, the expression of E-selectin increasel. As the time of infection went by, the expression of E-selectin gradually decreased. When the cytomegalovirus infection lasted 48 hours, the expression of E-selectin returned to the fundamental level. There were significant differences among the groups at 0, 4, 8, 12, 24 and 48 hours （ P 〈 0.01 ）. Conclusions： The infection with HCMV can enhance the expression of E-selectin mRNA in HUVEC. So we can say that HCMV may induce the inflammatory reaction by up-regulating the E-selectin expression on endothelial cells, which facilitates the occurrence and development of AS.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第2期124-126,共3页
Journal of Shandong University:Health Sciences
基金
山东大学大学生科技创新基金项目
关键词
人类巨细胞病毒
人脐静脉内皮细胞
E选择素
Human cytomegalovirus
Human umbilical vein endothelial cells
E-selectin
