摘要
目的了解毒淋巴T细胞相关抗原-4(CTLA-4)基因外显子1多态性与自身免疫性多腺体综合征Ⅲ型(APSⅢ)的关系。方法采用PCR-RFLP法检测52例正常对照者,39例经典1型糖尿病(T1DMA)患者和16例APSⅢ临床及亚临床患者CTLA-4基因外显子1的多态性。结果正常对照组CTLA-4外显子A等位基因出现频率0.212,低于G等位基因出现频率0.788。正常对照组不同基因型及等位基因均显示女性的GG基因型或G等位基因均较男性高,但差异均无统计学意义(χ2分别为2.856和0.003,P为0.24和0.352)。3组的A、G等位基因频率比较:χ2值和P值分别为1.476和0.478。结论G是本组人群中的主体基因。A基因频率在正常对照组、T1DMA组和APSⅢ组之间呈依次上升的趋势,推测A基因与疾病易感可能有相关性。同时表明CTLA-4仅仅是自身免疫疾病发生的次要因素。
Objective To learn the relationship between CTLA-4 exon 1 49A/G and APS Ⅲ. Methods 52 normal control, 39 T1DMA cases and 16 APS m cases. Results The frequency of allele G, 0.788 was higher than A, 0.212. GG genotype and G allele was higher in NC group. But it was no significant difference（x^2 =2.856, P =0.24;x^2 =0. 003, P =0.352）. G and A alleles in 3 groups was no significant difference too. Conclusion The main allele is G in the research groups. The CTLA-4 polymorphism has race difference. There is a increased trend of A allele in NC, T1DM and APS Ⅲ. So there may be a relation between A allele and susceptibility of autoimmune disease.
出处
《首都医科大学学报》
CAS
2007年第1期97-100,共4页
Journal of Capital Medical University
基金
首都医学发展科研基金(2002-2005)
北京市科委(H030930030430)资助项目
