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17β-雌二醇下调人成骨样MG63细胞结缔组织生长因子表达机制的研究

Investigation on the mechanism of 17β-estradiol-downregulating connective tissue growth factor expression in human osteoblastic MG63 cells
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摘要 10μmol／L雌二醇可明显下调人成骨样MG63细胞结缔组织生长因子（CTGF）。RNA及蛋白的表达；蛋白激酶A（PKA）阻断剂H-89可完全阻断雌二醇对CTGF的降调节作用。PKA活化剂forskolin可模拟雌二醇作用，明显下调人成骨样MG63细胞CTGF mRNA、蛋白的表达。17β-雌二醇下调人成骨样MG63细胞CTGF表达由PKA介导。 17βestradiol （10-s tool/L） decreased the expression of CTGF mRNA and protein, which could be completely blocked by H-89, protein kinase A （PKA） antagonist. Forskolin （ PKA agonist） can mimic the effect of estradiol on CTGF expression and reduce the expression of CTGF. 17β-estradiol can inhibit the CTGF expression via PKA pathway in human osteoblastic-like MG63 cells.
作者 隋国良 彭依群 翟木绪 何玉玲 廖二元
机构地区 中南大学附属湘雅二医院代谢内分泌研究所
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2007年第1期74-76,共3页 Chinese Journal of Endocrinology and Metabolism
基金 国家自然科学基金资助（30572078,30600661）
关键词 雌二醇 结缔组织生长因子 成骨样MG63细胞 Estradiol Connective tissue growth factor Osteoblastic-like MG63 cells
分类号 R686 [医药卫生—骨科学]
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参考文献11

  • 1Brigstock DR.The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) family.Endocrine Rev,1999,20:189 -206. 被引量：1
  • 2彭依群,廖二元,邓小戈.雌二醇诱导人成骨样细胞差异表达基因筛查[J].中华内科杂志,2003,42(8):561-565. 被引量：7
  • 3Nishida T,Nakanishi T,Asano M,et al.Effects of CTGF/Hcs24,a hypertrophic chondrocyte-specific gene product,on the proliferation and differentiation of osteoblastic cells in vitro.J Cell Physiol,2000,184:197-206. 被引量：1
  • 4Chen Y,Blom IE,Sa S,et al.CTGF expression in mesangial cells:involvement of SMADs,MAP kinase,and PKC.Kidney Int,2002,62:1149-1159. 被引量：1
  • 5Suzuma K,Naruse K,Suzuma I,et al.Vascular endothelial growth factor induces expression of connective tissue growth factor via KDR,Flt1,and phosphatidylinositol 3-kinase-akt-dependent pathways in retinal vascular cells.J Biol Chem,2000,275:40725-40731. 被引量：1
  • 6Riewald M,Kravchenko VV,Petrovan RJ,et al.Gene induction by coagulation factor Xa is mediated by activation of protease-activated receptor 1.Blood,2001,97:3109-3116. 被引量：1
  • 7Abraham DJ,Shiwen X,Black CM,et al.Tumor necrosis factor-α suppresses the induction of connective tissue growth factor by transforming growth factor-β in normal and scleroderma fibroblasts.J Biol Chem,2000,275:15220-15225. 被引量：1
  • 8Stratton R,Raljkumar V,Ponticos M,et al.Prostacyclin derivatives prevent the fibrotic response to TGF-β by inhibiting the Ras/MEK/ERK pathway.FASEB J,2002,16:1949-1951. 被引量：1
  • 9Liu J,Kosma VM,Vanttinen T,et al.Gonadotrophins inhibit the expression of insulin-like growth factor binding protein-related protein-2 mRNA in cultured human granulosa-luteal cells.Mol Hum Reprod,2002,8:136-141. 被引量：1
  • 10Fan WH,Kamovsky MJ.Activation of protein kinase C inhibits the expression of connective tissue growth factor.Biochem Biophys Res Commun,2000,275:312-321. 被引量：1

二级参考文献14

  • 1Velculescu V E.Serial analysis of gene expression[J],1995(5235). 被引量：5
  • 2Yang GP;Ross DT;Kuang WW.Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes[J],1999(6). 被引量：2
  • 3Lander ES;Linton LM;Birren B.Initial sequencing and analysis of the human genome[J],2001(6822). 被引量：4
  • 4Riggs BL;Khosla S;Melton LJ 3rd.A unitary model for involutional osteoporosis: estrogen deficiency causes both type I and type II osteoporosis in postmenopausal women and contributes to bone loss in aging men[J],1998. 被引量：1
  • 5Liao EY;Luo XH;Deng XG.Effects of 17beta-estradiol on the expression of membrane type 1 matrix metalloproteinase (MT1-MMP) and MMP-2 in human osteoblastic MG-63 cell cultures[J],2001(24). 被引量：1
  • 6Hubank M;Schatz DG.Identifying differences in mRNA expression by representational difference analysis of cDNA[J],1994. 被引量：1
  • 7Lisitsyn N;Lisitsyn N;Wigler M.Cloning the differences between two complex genomes[J],1993. 被引量：1
  • 8Pastorian K;Hawel L 3rd;Byus CV.Optimization of cDNA representational difference analysis for the identification of differentially expressed mRNAs[J],2000. 被引量：1
  • 9Yancopoulos GD;Oltz EM;Rathbun G.Isolation of coordinately regulated genes that are expressed in discrete stages of B-cell development,1990. 被引量：1
  • 10Liang P;Pardee AB.Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction[J],1992. 被引量：1

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中华内分泌代谢杂志
2007年 第1期

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