摘要
目的:观察针对人端粒酶RNA模板区的核酶对肝癌细胞端粒酶活性和细胞凋亡的影响。方法:利用脂质体Lipofectamine介导,将已构建好的带有端粒酶核酶基因的重组质粒pBBS212Rz及空载质粒pBBS212转染肝癌SMMC-7721细胞,采用TRAP-ELISA法检测端粒酶活性,用倒置相差显微镜及流式细胞仪观察细胞生长和凋亡情况。结果:重组质粒pBBS212Rz转染的肝癌SMMC-7721细胞的端粒酶活性明显下降,细胞生长速度明显变慢,凋亡加速。结论:端粒酶核酶对肝癌细胞端粒酶活性和细胞生长有抑制作用,可望成为肝癌基因治疗的方法。
Objective： To observe the effects of the ribozyme targeting the human telomerase RNA （hTR） on telomerase activity and cell apoptosis of human hepatocellular cancer cell line SMMC. 7721. Methods： The eucaryotic expression vector pBBS212Rz and vacant plasmid pBBS212 as a control were transfected respectively into the human hepatocellular cancer cell line SMMC. 7721 by lipofectamine. Telomerase activity in transfected ceils was detected by TRAP-ELISA （Telomeric Repeat Amplification Protocol and enzyme-linked immunosorbant assay）, Cell proliferation and apoptosis were detected by phase contrast microscope and flow cytometry. Results ： Telomerase activity in ribozyme transfected group decreased obviously compared with the control group, Cell proliferation became slow obviously and cell apoptosis was accelerated. Conclusions： Those findings suggested that the hammerhead ribozyme targeting the hTR template can inhibit telomerase activity of hepatocellular carcinoma in vitro and it will be a new therapeutic approach to hepatocellular carcinoma.
出处
《陕西医学杂志》
CAS
北大核心
2007年第3期286-288,共3页
Shaanxi Medical Journal
