摘要
目的:观察D-半乳糖衰老大鼠血清睾酮含量及睾丸生精细胞p53基因表达的变化。方法:20只SD大鼠随机分为正常组、衰老模型组,每组均为10只。采用D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。ELISA方法检测血清睾酮含量,SP免疫组化法检测睾丸生精细胞p53基因的表达。结果:衰老大鼠血清睾酮含量明显下降(与正常大鼠相比:P＜0．01);衰老大鼠睾丸p53阳性细胞率明显高于正常大鼠(P＜0．01)。结论:衰老大鼠睾丸生精细胞p53基因表达上调,睾丸功能减退。
Objective： To observe the changes of testosterone content in serum and p53 gene expression of spermatogenic cells in testis of D-gal aging rats. Methods：20 SD rats were applied in this study and divided into 2 groups at random： normal group, aging model group, and each group had 10 rats. The sub-acutely aging model rats were made by injecting D-gal into abdominal cavity continually. ELISA was used to detect the testosterone content in serum, SP immunohistochemistry to observe the p53 expression of spermatogenic cells.Results： The testosterone content of aging rats decreased significantly compared with normal rats （P 〈0.01）; the p53 positive cell rate of aging rats were obviously higher than normal rats （P〈0.01）. Conclusions： The p53 gene expression of spermatogenic cells in testis of aging rats increased, and the function of testis decline.
出处
《承德医学院学报》
2007年第1期7-9,F0002,共4页
Journal of Chengde Medical University
基金
承德市科技局项目(200411032)
