摘要
目的探讨白细胞介素-18（IL-18）、白细胞介素-12（IL-12）和干扰素-γ（INF-γ）在儿童1型糖尿病酮症酸中毒（DKA）中的作用。方法采用酶联免疫吸附（EUSA）方法检测61例儿童1型糖尿病（T1DM）患儿（其中28例伴DKA，33例无DKA）和30例正常对照者IL-18、IFN-γ和IL-12的血清水平。结果（1）T1DM患儿中血清IL-18水平明显比对照组高[（691．99±381．42）pg／ml vs （310．02±265．32）pg／ml，P=0．03]，而两组间IFN-γ和IL-12水平则无差别。（2）DKA组患儿中IL-18和IFN-γ血清水平明显高于无DKA组[（759．25±353．82）pg/ml vs（634.93±399．79）pg/ml，P=0．001和（9．35±8．06）pg/ml vs （5．21±6．47）pg／ml，P=0．044]，而两组间IL-12血清水平则无差别。（3）IL-18和IFN-γ呈相关趋势，而与IL-12正相关。结论DKA患儿IL-18和IFN-γ血清水平明显升高，提示其可能在DKA的发生、发展中起重要作用。
Objective To investigate the effect of the serum interleuldn （IL） -18, IL-12 and interferon （IFN） -γ levels in pediatric patients with diabetic ketoacidosis （DKA）. Method Sixty-one type 1 diabetic mellitus （T1DM） pediatric patients （28 with DKA , 33 without DKA） and 30 healthy controls were recruited in this study. Serum IL-18, IL-12, and IFN-T levels were measured in both groups by enzyme linked immunosorbent assay （ELISA）. Results Serum IL-18 and IFN-γ levels were significantly higher in patients with DKA than those in patients without DKA [ （759.25 ± 353.82） pg/ml vs. （634.93 ± 399.79） pg/ml, P = 0.001 and （9.35 ± 8.06） pg/ml vs （5.21 ± 6.47） pg/ml, P = 0.044, respectively]. Moreover, serum IL-18 levels were significantly higher in patients than those in controls [ （691.99 ± 381.42） pg/ml vs （310.02 ± 265.32） pg/ml, P = 0.03]. The serum IL-12 and IFN-y levels were not different between patients and controls [ （277.49 ± 206.99） pg/ml vs （351.40± 223.45） pg/ml, P = 0.446, and （7.02 ± 7.53） pg/ml vs. （5.59 ± 5.34） pg/ml, P = 0.215, respectively]. Serum IL-18 levels were correlated positively with serum IL-12 levels （ r = 0.254, P = 0.014） and very likely correlated positively with serum IFN-γ levels （ r = 0. 193, P = 0.064）. Conclusions Serum IL- 18 and IFN-γ levels were increased in patients with DKA compared with patients without DKA, and could serve as a predictor of process of DKA.
出处
《中华急诊医学杂志》
CAS
CSCD
2007年第2期193-195,共3页
Chinese Journal of Emergency Medicine
基金
浙江省科技厅资助项目（2005C33027）,浙江省卫生厅资助项目（2005A062）
