摘要
目的:探讨乙型肝炎病毒(HBV)二对半抗原抗体系统、前S1蛋白(Pre-S1)和HBV脱氧核糖核酸(HBV-DNA)之间的关系。方法:用酶联免疫吸附试验(ELISA)法检测209份HBV感染者血清的HBV二对半抗原抗体系统,实时荧光定量聚合酶链反应(FQ-PCR)检测HBV DNA。结果:HBV-DNA滴度小于每毫升1×105cop ies者35份(16.7%),HBV-DNA滴度在1×105-1×107cop ies者88份(42.1%),HBV DNA滴度大于1×107cop ies者86份(41.1%)。HBV-DNA滴度与HBeAg阳性率、Pre-S1阳性率呈正相关,相关系数分别为0.998(P=0.039)和0.882(P=0.024)。结论:HBeAg和Pre-S1也是反应HBV复制的指标。对HBeAg阴性的慢性乙型肝炎患者检测Pre-S1,可帮助判断HBV复制情况。
Objective： To explore the internal relations between expressional patterns of serological markers of hepatitis B virus （HBV） and HBV deoxyribonucleic acid （DNA）. Methods： Immunological markers and HBV DNA of 209 serum samples from HBV infected people were detected by using ELISA and real-time fluorescence quantitative polymerase chain reaction （FQ-PCR）. Results： Among the 209 samples, 35 （16.7%） had HBV-DNA titres lower than 1 ×10^5 copies/ml, 88 （42.1%） had the titre of 1 ×10^5 - 1 ×10^7 copies/ml, and 86 （41.1%） had the titre higher than 1 ×10^7 copies/ml respectively. HBV-DNA titer was positively correlated with positive rate of HBeAg and positive rate of HBV pre-S1 protein （ Pre-S1 ）. The correlation coefficients were 0. 998 （ P = 0. 039 ） and 0. 882 （ P = 0. 024） respectively. Conclusions： Both HBeAg and Pre-S1 are indexes of the replication of HBV to a certain extent. In chronic hepatitis B cases with negative HbeAg, test of Pre-S1 can help to judge the replication of HBV.
出处
《贵阳医学院学报》
CAS
2007年第1期45-47,共3页
Journal of Guiyang Medical College
基金
贵州省省长基金资助项目(黔省专合字2005-223)
关键词
肝炎病毒
乙型
肝炎抗体
乙型
肝炎抗原
乙型
聚合酶链反应
hepatitis B virus
hepatitis B antibodies
hepatitis B antigens
polymerase chain reaction
