摘要
采用3,5-二硝基水杨酸(DNS)法对内蒙古毛乌素沙地碱湖的钝顶螺旋藻S1与国外引进的钝顶螺旋藻S2和极大螺旋藻S3的淀粉酶(AMY)活性,及温度和pH值对其影响进行了初步的比较研究。结果表明:S1、S2和S3的AMY活性在25℃、pH5.6时分别为71.7U/gFW、44.1U/gFW和54.6U/gFW;最适温度均为40℃;最适值均为pH5.6。我国特有的S1AMY对温度和pH适应范围宽,且在低温、高温、强酸和强碱下的活性比引进种的高。
A preliminary study on dynamics of the amylase （AMY） and influence of temperature and pH on AMY activity from the domestic Spirulina（Arthrospira） platensis （ S1 ） from alkaline lake in Mu Us Sandy Land and the imported S. （A.）platensis （S2） and S. （A.） maxima （S3 ） as well made by means of DNS. The results showed that activities （25℃ ,PhS. 6） of S1, S2 and S3 is 71.7 U/g FW,44. 1U/g FW and 54.6 U/g FW value is respectively;Optimum temperatures is 40℃ ; Optimum pH 5.6. Adaptive range of S1 AMY which is endemic in China for temperature and pH value are wide, and activity under low and high temperature as well as strong alkali conditions is higher than that of imported species.
出处
《科学技术与工程》
2007年第7期1300-1304,共5页
Science Technology and Engineering
基金
国家自然科学基金项目(39960062)
内蒙古自然科学基金项目(990303-4)资助
关键词
螺旋藻
淀粉酶
活性
温度
PH值
Spirulina（Arthrospira） amylase （AMY） activitg temperatare pH value
