摘要
应用厌氧Hungate技术,从大庆油田常规污水回注工艺采油的采出液中分离到一株聚丙烯酰胺降解菌株A9。对该菌株进行形态、生理生化、分子生物学鉴定结果表明:菌株为革兰氏阳性(G+),短杆状,具有硫酸盐还原功能,产H2S,兼性厌氧,通过核糖体16S rDNA基因序列鉴定,菌株与Anaerofilum pentosovorans(AP716816S)的相似性为98%,暂时命名为Anaerofilum pen-tosovoransA9。扫描电镜和红外光谱分析结果表明:菌株以聚丙烯酰胺为唯一碳源,菌株作用前后表面结构发生变化,分子链上的酰胺基水解成羧基,侧链降解,部分官能团发生改变,浓度为500mg/L时,20 d菌株生物降解率为61.2%,其溶液粘度下降显著。气质联机(GC-MS)初步分析表明:聚合物发生断链生成的低分子量化合物除含双键、环氧和羰基的聚丙烯酰胺碎片外,大多属于一般丙烯酰胺低聚体的衍生物。
A hydrolyzed polyacrylamide （HPAM）-degrading bacteria strain A9 was isolated, using the Hungate anaerobic technique, from an extraction fluid sample of the Daqing Oil Field. The isolates was gram-positive, short bacillus,anaerobic bacteria capable of reducing sulfate to H2 S. The bacteria strain was named Anaerofilurn pentosovorans A9 because its alignment of 16S rDNA sequence had 98% similarity with those of A9 and Anaerofilum pentosovorans （AP716816S）. Living on HPAM as the sole source of carbon,the bacteria changed polymer＇s surface structure,hydrolyzed amide to carboxyl group by molecule chain amido hydrolysis, degraded the side-chain, and altered some functional groups. 61. 2% of HPAM （500 mg/L） was degraded in 20 days;in the mean time,viscosity of the solution was significantly reduced. The degradation products were polyacrylamide fragments with double bond, epoxy, carbonyl group, and many acrylamide oligomer derivatives.
出处
《华东理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2007年第1期57-60,103,共5页
Journal of East China University of Science and Technology
基金
国家自然科学基金重大国际合作项目(50521140075)
