摘要
目的探讨兔肺缺血-再灌注损伤时左旋精氨酸对氧化应激、降钙素基因相关肽(CGRP)的影响。方法采用在体兔的缺血-再灌注损伤动物模型。雄性日本大耳兔30只,随机分成3组,每组10只:假手术对照组(S组)、缺血再灌注组(IR组)、左旋精氨酸+缺血再灌注组(L-Arg组)。分别在开胸前、缺血末、再灌注末从左颈外静脉采血,检测各组丙二醛(MDA)、一氧化氮(NO)和超氧化物歧化酶(SOD)活力和CGRP含量。结果L-Arg组在缺血末及再灌注末SOD活力及NO含量明显高于S组及IR组(均P<0.01),而IR组缺血末及再灌注末与S组比较均有所下降(均P<0.05)。在再灌注末,IR组MDA含量明显高于S组和L-Arg组(均P<0.05),而CGRP含量却明显低于S组和L-Arg组(均P<0.01)。结论左旋精氨酸可能通过诱导SOD和CGRP的生成对兔肺组织的缺血-再灌注损伤起保护作用。
Objective To probe into the effect of L-arginine on the rabbit lung injured by ischemia/reperfusion. Methods Thirty male rabbits were randomly divided into three equal groups ： the sham operation group （ group S）, ischemia-reperfusion group （ group IR） and L-arginine ＋ ischemia-reperfusion group （ group L-Arg ＋ IR）. Rabbits of the L-Arg ＋ IR group were given each an Ⅳ injection of 100 mg· kg^-1 of L-arginine. A model of ischemia-reperfusion injury of the left lung was then set up in each of the rabbits of group IR and group L-Arg ＋ IR group by clamping（60 min） and declamping（60 min） of the hilus of the left lung under Ⅳ urethan anesthesia and mechanical ventilation. The same operation was performed on each of the animals of group S except for that the hilus of the left lung was not subjected to damping/declaming. Venous blood samples from each of the rabbits of the 3 groups were taken before the operation, at the termination of ischemia （ group IR and group L-Arg ＋ IR） or 60 min after the operation （group S） and at the termination of reperfusion （group IR and group L-Arg ＋ IR） or 120 min after the operation （ group S）, respectively, for the determination of the activity of plasma superoxide dismutase （ SOD）, content of plasma malonyldialdehyde （ MDA ）, nitric oxide （NO） and calcitonin gene-related peptide （CGRP）. Results At the termination of ischemia and reperfusion, the activity of SOD and content of NO in rabbits of the L-Arg ＋ IR group were significantly higher than those in animals of group S or group IR（P 〈 0. 01 ,P 〈 0.01 ）, while the activity of SOD and content of NO were significantly lower in rabbits of group IR than those in animals of group S（ P〈0. 05,P 〈0.05）. At the termination of reperfusion, the content of MDA in rabbits of group IR was obviously higher than that in those of group S and group Larg ＋ IR while the content of CGRP was significantly lower than that in rabbits of group S and group L-Arg ＋ IR（
出处
《医药导报》
CAS
2007年第2期127-129,共3页
Herald of Medicine
