摘要
取出生30 d以内的山羊(Capra hircus)羔,分离其肠道淋巴集结,经匀浆、反复冻融、离心,制备提取物。用该提取物饲喂体质量(21.2±1.1)g的淡水白鲳(Colossoma brachypomum)以检验其对鱼类免疫力及生长的影响。实验组和对照组各设3个平行组,每个平行组有29尾鱼,饲于1个0.3 m3水族箱。实验组按质量比为2%的比例向基础饲料中添加提取物,对照组只投喂基础饲料。15 d后,实验组淡水白鲳的白细胞吞噬活性、血清抗菌活力、血清溶菌酶活性、血清溶血素、特定生长率、饵料系数均显著高于对照组(P<0.05);血清酚氧化酶和超氧化物歧化酶活性与对照组相比没有显著提高(P>0.05)。实验表明,山羊羔肠道淋巴集结提取物能提高淡水白鲳免疫力,并对其生长有明显的促进作用,其作为饲料添加剂具有良好的应用前景。
Goat lamb intestinal Peyer＇s patches are both central and peripheral immune organ where B-lymphocytes originate, develop and maturate. Previous study has shown that the goat lamb Peyer＇ s patches consist of active stimulus to immune system of chicken and rabbit kids. In the present research, a study was conducted to determine effect of goat lamb intestinal Peyer＇s patches extract on immunity and growth of limnetic fish Colossoma brachypomurn. Peyer＇s patches extract was obtained by centrifugation isolation after tissue homogenizing and repeating freezing-and-thaw of intestinal Peyer＇ s patches from goat lamb not beyond 30 days after birth. One hundred and seventy-four C. brachypomum with （21.2 ± 1.1） g initial body weight were divided into test group and control group evenly. In each group 87 fish were divided again into three parallel subgroups. Every subgroup contained 29 fish which were kept in an indoor circular aquariurn system（0.3 m^3） for 15 d. The diets of test group included 2% goat lamb Peyer＇s patches extract（total protein 20 mg/mL in PBS solution） while those of control group did not. At the end of experiment period several immune indices were tested. Results showed that adding Peyer＇s patches extract to the daily feed promoted immunoresponse level, including phagocytic activity of phagocytes, serum antibacterial activity, serum hemolysin level and serum baeteriolytic activity significantly（ P〈0.05） in C. brachypomum. Phagocytic activity of phagocytes was 52.33% in test group and 20.67% in control group,increasing by 153%. Serum antibacterial activity was 0.68 in test and 0.33 in control, increasing by 104 %. Serum bacteriolytic activity was 0.15 in test group and 0.12 in control group, increasing by 25 %. Serum hemolysin value was 8.67 in test group and 4.33 in control group, increasing by 100 %. However, the increases of phenoloxidase （PO） and superoxide dismutase （SOD） activities were not remarkable （ P 〈 0.05 ）. Meanwhile, relative growth rate of fish
出处
《中国水产科学》
CAS
CSCD
北大核心
2007年第2期290-294,共5页
Journal of Fishery Sciences of China
基金
山东省科技攻关项目(2005GG320801)
山东省优秀中青年科学家科研奖励基金(02BS040)
