摘要
目的观察S180小鼠皮下移植瘤组织淋巴管的形态学改变,为进一步研究肿瘤淋巴管转移机制提供形态学依据。方法将小鼠S180腹水型瘤细胞接种于昆明小鼠左腋部皮下,20天后切取肿瘤及瘤周组织和对侧正常皮肤,分别用HE染色确认肿瘤,半薄切片筛选淋巴管并观察,超薄切片透射电镜观察淋巴管形态。结果2周以上小鼠腋部可见明显肿块,致癌率达100％。移植瘤中心区液化坏死,无毛细淋巴管。周边区淋巴管检出率明显高于正常侧且管腔大,形态不规则,管壁薄。内皮细胞肿胀,高尔基体、粗面内质网及溶酶体等细胞器的形态有改变。结论S180移植肉瘤中心区无淋巴管,瘤周边区淋巴管密度增多,管腔扩张,形态不规则,伴有内皮细胞损伤。
Objective To provide the morphological base for further studying the mechanism of tumor metastasis, we observed morphological changes of lymphatic vessels in transplant subcutaneous sarcoma of S180 mice. Methods S180 cells were inoculated subcutaneously into axillary fossae of KM mice. Tumor tissues, tumor peripheral tissue,and normal tissue in opposite side were obtained after 20 days of inoculation. HE staining was used to observe the development and invasion of tumor, semi-thin section was used to detect the lymph vessels of the samples, and ultrathin sections under transmission electron microscope was used to observe the ultrastructure of lymph vessels. Results The animal model succcessfuUy showed that obvious masses could be seen subcuteously in the axillary fossae two weeks after innoculation. Necrosis showed in the center region and no lymphatic vessels in the center of the tumor could be seen. The number of lymphatic vessels in the peripheral tissue was higher than that in the normal side. The lymphatic lumina were dilated and irregular, and wall of the lymph vessel was thin. Under electron microscope, the organelle of endothelial cells showed morphological changes of Golgi bodies, rough endoplasmic reticulums, and lysosomes. Conclusion There is no lymphatic vessel in the center of S180 subcutaneous sarcoma, the number＇of the lymphatic vessels increased around the tumor, the cavity extended in irregular shapes, and the endothelial cells of lymphatic vessel damaged.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2007年第1期23-25,共3页
Journal of Harbin Medical University
关键词
淋巴管
小鼠
S180皮下移植瘤
lymphatic vessel
mice
S180 transplant subcutaneous sarcoma
