摘要
采用Ｆｏｇａｒｔｙ导管损伤大鼠胸主动脉段内皮细胞，观察１、５、１５和２０天后胸主动脉组织形态学改变；提取胸主动脉段组织ＲＮＡ采用反转录－多聚酶链反应方法测定原生型一氧化氨合成酶基因和内皮素－１基因ｍＲＮＡ的表达。结果表明，胸主动脉压血管平滑肌细胞在内皮损伤后增殖，内皮素－１基因表达增加，原生型一氧化氮合成酶基因表达减少，提示原生型一氧化氮合成酶基因和内皮素－１基因表达的改变可能在内皮损伤后的平滑肌细胞增殖中起重要作用。
Aim This study is to investigate the changes ofconstitutive nitric oxide synthetase(cNOS )gene and endothelin -1(ET-1)gene expression after endothelial injury.Methods Endothelial cells of rats'thoracic aortaewere injuried by Fogarty balloon catheter. Thoracic.aortae tissues were under histological examination at 1,5,15 and 20 days after endothelial injury.The cNOS gene and ET-1 gene mRNA expressions in thoracic aortae tissues at different time stage were determined by reverse transcription polymerase chain reaction(RT-PCR)methods.Results Histological examination showed smooth muscle cell(SMC)proliferation and intimal thickening in thoracic aortae at 5,15 and 20 days after endothelialinjury.Some sites of intima began to reendothelialize at 20 days.The ET-1 gene expression in thoracic aortae tissue increased significantly at l and 5 days,and s still maintained at higher level at 15 and 20 days than control.To the opposite,the cNOS gene expression disappeared at 1 day,and was lower than control at 20 days.Conclusion The results indicated that alterations of ET-1 gene and cNOS gene expression were associated with SMC proliferation in this endothelial injury model.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第3期190-193,共4页
Chinese Journal of Arteriosclerosis
关键词
内皮损伤
内皮素
一氧化氮合成酶
动脉粥样硬化
Endothelial injury
Smooth
muscle cell
Constitutive nitric oxide synthetase
Endothelin-1
Gene expression
