摘要
目的探讨血管内皮祖细胞(EPC)局部移植防治血管成形术后再狭窄形成的可行性。方法分离、鉴定并培养新西兰大白兔外周血(EPC),用2．5F球囊扩张并损伤兔右侧颈动脉血管内皮,对损伤血管进行局部EPC移植。共作细胞移植兔13只,其中3只移植荧光标记EPC;对照组8只,局部灌注生理盐水。细胞移植后4 d,对2只荧光标记细胞移植兔取移植细胞后受损伤血管行病理组织学检查,其余实验兔4周后对损伤血管行病理组织学检查。结果荧光标记细胞移植后4d,病理学检测显示损伤血管内皮有强荧光表达;4周后,细胞移植组血管壁轻度增厚,对照组血管壁增厚明显,血管腔明显狭窄。两组间血管壁厚度差异有统计学意义(P＜0．01)。结论介入法局部移植同种异体血管内皮祖细胞可防治血管成形术后再狭窄的形成。
Objective To investigate homografting vascular endothelial progenitor cells （EPCs） for preventing restenosis formation of carotid artery in New Zealand white rabbit models. Methods EPCs of New Zealand white rabbits were isolated, confirmed and expanded though the injured carotid arterial endothelium of rabbit model induced by dilatation with a 2.5 F balloon; and then EPCs were transplanted into the injured endothelium of the cells transplantation group （n = 13, 3 of them were transplanted with fluorencently-labeled-EPCs）, while equal volume of saline without EPCs was injected into the injured endothelium in the control group （n = 8）. Histopathology was performed at 4 days after transplantation for the 2 rabhits, with fluorencently-labeled-EPCs. All of the rest remained rabbits were killed 4 weeks later for histological examinations. Results The histopathological slides showed that the fluorescence-positive expression existed in the injured endothelium 4 days after transplantation. At 4 weeks after the EPCs transplantation, there were less restenosis and less vascular wall thickening in the rabbits of cells transplantation group than those of the control group （P 〈 0.01）. Conclusion The local interventional homografting heterogeneous endothelial progenitor cells can prevent restenosis after the carotid artery angioplasty in New Zealand White rabbit model.
出处
《介入放射学杂志》
CSCD
2007年第2期95-98,共4页
Journal of Interventional Radiology
基金
东南大学优秀博士学位论文基金(200720)
关键词
血管内皮祖细胞
再狭窄
细胞移植
兔
Endothelial progenitor cells
Restenosis
Cells transplantation
Rabbits
