摘要
目的:掌握河北省食品中大肠杆菌O157:H7分布状况及菌株的毒力研究。方法:运用免疫磁珠富集进行分离培养,并用PCR进行毒力基因分析。结果:共采集食品530份,检出O157:H7大肠杆菌5株,总检出率为0.9%。其中,以生牛、羊肉检出率较高,分别为6.7%和4.4%。5株O157:H7菌株中,4株带有SLT2、eae和H ly 3种毒力基因,1株带有SLT1、SLT2、eae和H ly 4种毒力基因。结论:掌握食品中O157:H7分布及生物学特性对预防食源性疾病暴发有重要意义。
Objective：To detect the Escherichia coli O157：H7 positive rate of food and the toxic gene of the strain in Hebei Province. Methods：Escherichia coli O157：H7 was isolated with immune magnetic essay from foods and PCR was performed to detect the toxic gene of the strains. Results： Altogether 5 strains of O157 ： H7 were separated from 530 feces of different foods and the overall positive rate was 0. 9%. The positive rates of cattle and sheep was high and it was 6. 7% and 4. 4% respectively. For 5 strains,SLT1 ,SLT2, eaeA and lily toxic genes were detected,4 strains carried SLT2, eae and Hly genes, 1 strain carried SLT1, SLT2,eae and Hly genes. Conclusion：To enhance the surveillance of O157 ：H7 in food should be very important for the prevention and control of the foodborne disease outbreaks.
出处
《中国卫生检验杂志》
CAS
2007年第1期120-122,共3页
Chinese Journal of Health Laboratory Technology
