摘要
目的探讨益气化瘀方对糖尿病溃疡大鼠Smad3、Smad4表达的影响。方法将72只清洁级雄性SD大鼠分为正常创面组、糖尿病创面组、贝复济组、益气化瘀组、益气组和化瘀组。以糖尿病大鼠背部全层皮肤缺损开放性创面为模型,各组分别给予相应的处理措施后,应用免疫组化、图像分析、westernblot等技术,观察各组大鼠创面肉芽组织中Smad3、Smad4表达水平的变化。结果糖尿病创面组Smad3、Smad4的表达水平明显高于正常创面组(P<0.01),各中药组Smad3、Smad4的表达水平明显低于糖尿病创面组(P<0.01);Westernblot检测结果显示,益气化瘀组Smad3、Smad4的表达水平明显低于益气组和化瘀组(P<0.01),化瘀组Smad4的表达水平低于益气组(P<0.05)。结论糖尿病创面难愈合与Smad3、Smad4的高表达有关;益气化瘀中药能明显促进糖尿病溃疡大鼠的创面愈合,下调Smad3、Smad4的表达水平是其作用机制之一。
Objective To explore the molecular mechanisms of Yiqi Huayu herbs on Smad3 and Smad4 in granulation tissue of diabetic ulcers rats. Methods Seventy-two male Sprague-Dawley （SD）rats with back full-thickness skin lesion in diabetic rats were randomly divided into 6 groups： Normal group, Untreated group, bFGF-treated group,Yiqi Huayu Herbs-treated group, Yiqi Herbs-treated group, Huayu Herbs-treated group.The protein expressions of Smad3 and Smad4 in granulation tissue of diabetic ulcers rats were tested with immunohistochemical methods, image analysis and Western blot. Result As compared with the Normal group,the expressions of Smad3 and Smad4 in Untreated group is lower （P 〈 0.01）o As compared with the Untreated group, the expressions of Smad3 and Smad4 in Chinese herbs treatment groups is lower （P〈0.01）.Westem blot were used to detect the expressions of Smad3 and Smad4,the expressions of Smad3 and Smad4 in Yiqi Huayu Herbs -treated group was lower than that in the Yiqi Herbs -treated group and Huayu Herbs - treated group（P〈0.01）,the expressions of Smad4 in Huayu Herbs Huayu Herbs -treated group than that in the Yiqi Herbs -treated group. Conclusion Yiqi Huayu herbs can promote the wound healing in rats. It was likely through reducing the expressions of Smad3 and Smad4.
出处
《上海中医药杂志》
北大核心
2007年第2期24-27,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市青年科技"启明星"培养计划资助项目(03QB14047)
上海市重点学科建设资助项目(T0304)
