摘要
目的通过失血性休克大鼠血液回输后造成的缺血-再灌注损伤(IRI)模型,研究肌肽对IRI后丙二醛(malondialdehyde,MDA)浓度、热休克蛋白70(HSP70)及炎性因子表达水平的影响。方法27只Wistar大鼠随机分成3组制作IRI模型,大鼠失血至40mmHg→维持20min→放置1h→全血回输→维持3h→处死。大鼠处死后取血浆检测MDA浓度,取肝、脑、肺、心、肾、脾组织制作石蜡切片,通过免疫组化染色比较肝、脑、肺、心、肾、脾组织切片HSP70阳性细胞数;取肝组织提取RNA比较肝组织HSP70及炎性因子mRNA表达量。结果与再灌损伤组相比,肌肽治疗组肝、脑、肺、心、肾、脾组织切片HSP70阳性细胞数及HSPa5、HSPalamRNA表达量明显升高;MDA浓度及IL-6、TNF-α、NF-κB1、SCYA2、SCYA3mRNA表达量明显降低。结论肌肽可以抑制IRI后MDA的生成、从mRNA水平促进HSP70的表达、抑制炎性因子mRNA的表达。
Objective To investigate the effect of carnosine on the concentration of malondialdehyde （ MDA）, expression of heat shock protein 70 （HSP70） and inflammatory factor after ischemia-re＇perfusion injury （IRI） in a Wistar rat model of ischemia-reperfusion injury. Methods Twenty-seven male Wistar rats were randomly divided into sham-operation group, mock-treated IRI group and camosin-treated IRI group. Animal ischemia was achieved by blood-letting and reperfusion injury was achieved by infusion of the bled blood. Rats of the shamoperation group were sacrificed after the operation. The mock-treated IRI group and carnosine- treated IRI group received 0. 9% sodium chloride solution and carnosine （90 mg/kg）, respectively, through femoral vein before reperfusion. All the bled blood was re-infused into the animals in 10 minutes and the rats were sacrificed 3 hours later. Blood samples were analyzed for serum MDA concentration. Liver, brain, lung, heart, kidney and spleen samples were taken for the examination of HSP70 expression. HSP70 and inflammatory factors＂ mRNA expression of hepatic cell of mock-treated IRI group and camosine-treated IRI group were measured and compared. expression of HSP70 in vital organs whereas MDA levels and inflammatory Results Compared with those in mock-treated IRI group the and HSPaS, HSPala mRNA in liver increased significantly, factors IL-6, TNF-α, NFkB1, SCYA2, SCYA3 mRNA in the camosine-treated group decreased significantly. Conclusion Camosine administration could promote the expression of HSP70 and suppress the increase of MDA and inflammatory factor mRNA expression.
出处
《医学分子生物学杂志》
CAS
CSCD
2007年第1期11-14,共4页
Journal of Medical Molecular Biology
