摘要
目的:观察牙囊发育不同时期串珠素(perlecan)分布、表达情况,探讨其与牙囊发育的关系。方法:出生0 d、1 d、1周、2周的SD大鼠取下颌第一磨牙及其牙囊组织,进行Perlecan免疫组化染色(SABC法),光镜观察其表达、分布,用图像分析测定其灰度值,并进行定量分析比较。结果:串珠素在各时间段牙囊均有阳性表达,而1 d、1周组表现为强阳性。图像分析结果表明1 d组、1周组大鼠阳性结果明显高于0 d组、2周组,出生后串珠素表达随着牙胚发育而不断增强,之后又随着牙胚的成熟而下降。结论:串珠素与牙囊的发育有关,在牙囊发育初期对牙囊基质的形成发挥作用,晚期随着基质的成熟其表达下降。
AIM：To observe the distribution and expression of perlecan in the dental follicle in the different developmental stages. METHODS： The dental follicle of the 1 st mandibular molar was taken from the 0 - day, 1 - day, 1 - week and 2 - week newborn rats. The samples were stained with the immunocytochemistry of SABC. The dis- tribution and expression of perlecan was analyzed with image analysis system. RESULTS.. Perlecan was expressed in the developmental stages of dental follicle. The results of image analysis showed that the positive expression of 1 - day and 1 - week samples was stronger than that of 0 - day and 2 - week samples. CONCLUSION ： The expression of perlecan is related to the development of dental germ and may play a role in the extralcellular matrix formation of the dental follicle in the primary developmental stages. Its expression reduces with the mature of dental germ matrix.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第1期1-3,共3页
Chinese Journal of Conservative Dentistry
关键词
串珠素
牙囊
免疫组化
牙胚
perlecan
dental follicle
immunocytochemistry
dental germ
