摘要
目的探讨长春地区戊型肝炎病毒(HEV)在人群中的感染情况及戊型肝炎病毒系统进化关系。方法用抗-HEV抗体试剂盒检测人血清中的抗体;对部分血清和粪便标本用逆转录聚合酶链式反应的方法(RT-PCR)检测HEV RNA,并对PCR阳性产物进行克隆测序,然后将序列进行分析。结果长春地区各年龄组共4944份人血清中有1127份为抗-HEV抗体阳性,阳性率为22.79%;其中男性阳性率为34.27%,女性阳性率为10.88%。20岁以下人群感染率为1.00%左右,自20岁起男性和女性分别以每年接近10.00%和3.00%的速度平稳上升。戊肝感染者中男性与女性的抗体阳性率之比为3.3:1,随着年龄上升,人群中戊肝感染者的抗体水平也缓慢上升。我们从急性戊肝患者血清中检测到1份HEV RNA阳性样本,从急性戊肝患者粪便中检测到2份为HEV RNA阳性样本,序列分析显示从我们克隆出的3株HEV的核苷酸序列的同源性为91.2-99.1%,该3株序列在ORF 2区438bp同1-4型间核苷酸同源性分别为:77.8%-82.3%、77.2%-78.1%、77.2%-99.1%、85.2%-95.2%;结论HEV在各年龄段人群中均有流行,但在年龄段20岁人群后的流行率呈逐年增长趋势,说明长春地区HEV感染主要发生在成年后。人感染的HEV的基因序列与猪群戊型肝炎病毒的基因Ⅳ型同源性最高。由进化关系看在长春地区人HEV与猪HEV不能分为不同的两支,说明长春地区人HEV和猪HEV很可能是由同一支分支进化而来。
Objective To investigate the prevalence and genotypes of HEV existed in persons in Changehun.Methods The anti-HEV antibody was detested in sera collected from people with anfi-HEV EIA. HEV RNA was detected by RT-PCR with ORF 2 primers. The positive PCR products were cloned and sequenced. The sequences were compared with thereported HEV isolates.Results 1127 d 4944 samples d people were positive for anfi-HEV antibody.The prevalence of HEV infection in the population was 22. 79%. The prevalence of male was significantly higher than that of female. The prevalence in persons with age below 20 yeats old was about 1.00% ,but in the persons with age above 20 years old,the prevalence in male and fence were stably rose with a speed of 10. 00% and 3.00% per year respectively. 1 sera samples of patient and 2 feces samples of patients were positive for HEV RNA . The sequence analysis showed that the identity at nucleofide level was 91.2 - 99.1% among them. They had 77.8 % - 82.3 % ,77.2 % - 78.1% ,77.2% - 99.1%, 85.2% - 95.2% identity to genotypes 1,2,3 and 4 respectively. Conclusion This study area is endemic of hepatitis E,the infection happened mainly among the adults with nearly 10.00% in male and 3.00% in female per year increasing. The prevalence in male of HEV is higher than in female. HEV sequences isolated from persons belong to HEV 4 and closest to isolates from pig in China. They belong to the same branch through the analysis of phylogeny.
出处
《中国实验诊断学》
2007年第2期201-204,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家"973"计划资助项目(2005CB23005)
