摘要
目的探讨利用外周血内皮祖细胞(EPCs)制备组织工程瓣膜的可行性。方法分离人外周血EPCs,采用酶-去垢剂法去除新鲜猪主动脉瓣细胞制备去细胞瓣膜支架,将培养的人外周血EPCs接种到去细胞瓣膜上。结果经酶-去垢剂法去除新鲜猪主动脉瓣细胞后,细胞成分全部去除,纤维支架保存完好。去细胞处理后瓣膜无明显细胞毒性。人外周血EPCs与去细胞瓣膜共孵育2周后,细胞紧贴瓣膜表面生长形成一层连续的单细胞层,初步生物力学测定示去细胞前与再内皮化后,瓣膜力学特性无明显改变。结论外周血分离培养扩增得到的EPCs能够再内皮化去细胞猪主动脉瓣构建组织工程瓣膜,外周血EPCs是组织工程瓣膜内皮种子细胞的新的来源。
Objective To investigate the feasibility of reendothelialization of decellularized porcine heart valves by endothelial progenitor cells in vitro. Methods Endothelial progenitor cells （EPCs） were isolated and expanded from human peripheral blood. The porcine aortic valves were decellularized by trypsin-EDTA,Triton X-100, RNase and DNase, and seeded by EPCs and cultured for two weeks. Results After treated with trypsin-EDTA,Triton X-100, RNase and DNase, the cells of porcine aortic valve were removed thoroughly, and the matrix integrity was preserved well. There were no significant cytotoxicity in decellularized valve, which was measured by MTY. HE stain and SEM revealed that a confluent monolayer cells formed on the surface of decellularized valve. Biomechanical test revealed that there was no signifcant difference between fresh valve and reendothelialized valve. Conculsion EPCs can be used to reendothelialize the decellularized porcine aortic valve to creat engineering heart valves. EPCs are a potential cell source of tissue engineering definitely.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2007年第1期110-115,共6页
Chinese Journal of Biomedical Engineering
基金
浙江省自然科学基金资助项目(M303648)
关键词
细胞培养
内皮祖细胞
组织工程瓣膜
cell culture
endothelial progenitor cells
tissue engineering heart valve
