摘要
采用超声波技术提取富硒绿茶功能成分,通过L9(34)正交试验对提取工艺进行优化,采用DPPH法和亚油酸体系法对富硒绿茶功能成分抗氧化活性进行了评价,并分析了化学组成。结果表明按照工艺:50%乙醇为溶剂,超声波功率250W,料液比为1:15,提取温度50℃,提取时间1.5h,重复提取两次,制得的富硒绿茶功能成分得率为43.76%,其抗氧化活性高于其他处理制得的提取物和阳性对照α-生育酚,其化学组成为:硒4.35±0.11μg/g,茶多酚(55.84±2.31)%,多糖(13.37±3.02)%,蛋白(25.75±4.05)%,咖啡碱(5.99±0.01)%。
The ultrasonic was used to extract Se-enriched green tea functional components. The extraction pararmeters were optimized by L9（3^4） orthogonal design. And the composition of Se-enriched tea functional components was researched. The antioxidant activity of Se-enriched green tea functional components was assessed by FTC method and DPPH method. The optimized parameters were as follow： 50% （W/V） ethanol as solvent, ultrasonic power of 250W, ratio of solid/liquid of 1：15, extracting for 2 times at 50℃ for 1.5 hours each time. The yield was 43.76%. The antioxidant activity of the extracts under the optimized condition, which contained Selenium 4.35 ±0.11μ g/g, tea polyphenol （55.84 ±2.31）%, polysaccharide （13.37 ± 3.02）%, protein （25.75 ±4.05）%, theine （5.99±0.01）%, was stronger than the extracts under other technology parameters and positive control α-tocopherol.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期142-147,共6页
Food Science
基金
国家自然科学基金项目(30671461)
教育部新世纪优秀人才支持计划(NECT-04-0501)
教育部优秀青年教师资助计划(EYTP-2003-355)
关键词
富硒绿茶
功能成分
超声波
抗氧化
Se-enriched
ultrasonic
functional components
antioxidant
