摘要
[目的]探讨持续性压力培养环境对兔骨髓基质干细胞增殖的影响,揭示持续压力致假体松动下沉进展及其术后活动影响的生物学机制。[方法]以新西兰兔的骨髓基质干细胞为实验材料,通过四唑盐(MTT)比色试验,观察压力对骨髓基质干细胞增殖的影响。[结果]骨髓基质干细胞在20、60、100 kPa持续性压力培养环境下MTT反应的OD值显著小于对照组,压力值越大,OD值越小。20、60、100 kPa持续性压力可显著抑制骨髓基质干细胞细胞增殖,抑制作用随压力值增大而增强。[结论]关节置换术后早期患者不宜过早下地,否则将产生持续性应力,可以引起骨髓腔内骨髓干细胞的抑制,不利于骨质愈合。且易产生假体下沉和松动。
[ Objective] To investigate the influence of pressure on the proliferation of rabbit bone marrow stromal cells （MSCs） and explain the relationship of the hip prosthesis submerge and loosening with continuous pressure after revision of total hip replacement. [ Method] A unit of MSCs pressure model was set up to load different pressures on MSCs cultured in vitro. [ Result] MSCs showed more proliferation capacity under discontinuous pressure. MTT assay was used to determine the cell proliferation of primarily euhured MSCs under discontinuous pressure. The MSCs OD value of pressured groups was much smaller than the control group at different times. Various magnitudes and durations of the discontinuous pressure could significantly suppress MSCs proliferation with the magnitude- dependent. [ Conclusion] At the early period after revision of total hip replacement, patients should lie and exercise on the bed until 6 weeks. Otherwise, the weight - bearing pressure may restrain MSCs proliferation which is harmful to union and may cause prosthesis submerge and loose.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2007年第2期141-143,共3页
Orthopedic Journal of China
基金
国家863课题(Z18-05-28)资助
关键词
骨髓基质干细胞
持续性压力
髋关节置换
细胞增殖
bone marrow stromal cells
continuous pressure
revision of total hip replacement
cell proliferation
