摘要
目的:探讨儿童急性白血病(AL)骨髓细胞中SODD、Bcl-2蛋白的表达及其与AL的分型、临床特征、疗效、预后的关系。方法:采用免疫组化SABC法检测86例AL患儿骨髓细胞SODD、Bcl-2蛋白表达情况。结果:初治组、难治复发组及完全缓解组SODD与Bcl-2蛋白阳性表达率明显高于对照组,差异有显著性(P<0.05),SODD、Bcl-2蛋白表达在ALL与ANLL间无显著差异(t=1.874,t=1.583,P>0.05);经化疗,完全缓解组SODD、Bcl-2阳性表达率均低于初治组,差异有显著性(t=2.054,t=2.703,P<0.05);难治复发组SODD蛋白表达显著高于初治组(t=-1.081,P<0.05),Bcl-2蛋白在初治组及难治复发组均高表达,差异无显著性(t=-1.196,P>0.05),但难治复发组Bcl-2阳性细胞百分率(45%～87%)显著高于初治组(5%～62%);等级相关分析显示SODD和Bcl-2蛋白表达呈显著正相关(P<0.01,r=0.726),经统计学分析,SODD及Bcl-2阴性表达者完全缓解率均高于阳性表达者,差异有显著性(P<0.05)。结论:SODD、Bcl-2均与AL的发生、发展、疗效及预后密切相关,且两者在AL的发生、发展过程有一定的协同作用。
Objective： To examine the expression of SODD and Bcl-2 proteins in bone marrow cells of acute leukemia （AL） in children and to detect a relationship, if any, with the classification, clinical features, therapeutic effects and prognosis of AL. Methods： Using the SABC method of immunohistochemical staining, the expression of SODD and Bcl-2 proteins in the bone marrow cells of 86 cases with AL was detected. Results： The positive expression rates of SODD and Bcl-2 in the first visit, relapse due to drug resistance and relief groups were obviously higher than that in the control group, with significant difference （P〈0.05）. There was no significant difference in the expression of SODD and Bcl-2 proteins between the acute lymphoblastic leukemia （ALL） and acute nonlymphoblastic leukemia （ANLL） groups （t=1.874, t=1.583, P〉0.05）. The positive expression rates of SODD and Bcl-2 in the pediatric patients with complete remission by chemotherapy were lower than that in the first visit pediatric patients, and there was a significant difference in the expressions （t=2.054, t=2.703, P〈0.05）. The expression of SODD protein in the refractory relapse group was higher than that in the initial treatment group （t=-1.081, P〈0.05）. High expression of bcl-2 protein occurred in both the first visit and refractory relapse groups, and there was no significant difference in the expressions （t=-1.196, P〉0.05）, whereas the percentage of bcl-2 positive cells in the refractory relapse group （45%-87%） was significantly higher compared to the first visit group （5%-62%） . The positive expression of SODD and bcl-2 proteins in the high risk （HR） group were all significantly higher than that in the standard risk （SR） group （t=-3.236, t=-3.555, P〈0.01）, and Pearson rank correlation analysis indicated that there was a positive correlation between the expression of SODD and bcl-2 proteins （P〈0.01, r=0.726）. In 30 pediatric pa- tients with AL in the initial treatment group, negativ
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第3期136-139,144,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金资助(编号:39970778)
