摘要
目的:探讨胆红素对抗急性肺损伤(ALI)形成的可能机制。方法:健康雌性W istar大鼠(190-210 g)30只,随机分为生理盐水对照组、脂多糖(LPS)致ALI模型组、胆红素干预组。检测肺组织匀浆中羟自由基(OH-)、过氧化氢(H2O2)和超氧阴离子自由基(O2.)含量以及肺组织中caspase-3表达的变化。结果:①ALI模型组肺组织匀浆OH-、H2O2、O2.含量及肺组织中caspase-3表达显著高于生理盐水对照组(均P<0.05)。②胆红素干预组肺组织匀浆OH-、H2O2、O2.及肺组织中caspase-3表达明显高于ALI正常大鼠(均P<0.05),但少于ALI模型组(均P<0.05)。结论:①胆红素能在一定程度上减少肺内凋亡细胞数量。②胆红素能减少ALI大鼠肺组织OH-、H2O2、O2.水平。③Caspase-3表达的变化有促脂多糖性肺损伤细胞凋亡作用。
AIM： To investigate the mechanisms by which bilirubin inhibits acute lung injury （ALI）. METHODS： 30 female Wistar rats were divided into normal group, ALI group and bilirubin treatment group. ALI was induced by intravenous injection of LPS. The contents of OH^-, H2O2 and O2^-· in the lung as well as the expression of caspase - 3 in the lungs were investigated. RESULTS ： （1） The contents of OH^-, H2O2 and O2^-· in the lung homogenate and the expression of caspase - 3 in the lungs in ALI group increased compared with those in normal group （ P 〈 0. 05 ）. （ 2 ） The contents of OH^- , H2O2 and O2^-· in the lung homogenate and the expression of caspase - 3 in the lungs in bilirubin treatment group increased compared with those in normal group, but decreased compared with those in ALI models （ P 〈 0. 05 ）. CONCLUSION： （ 1 ） Bilirubin was shown to be able to ameliorate apoptosis in ALI rats. （2） The increase in the contents of OH^-, H2O2, O2^-· in ALI group indicated the development of oxidative lung injury, which was ameliorated by bilirubin. （3） Expression of caspase - 3 confirmed that the model made by LPS was associated with apoptosis, which was reduced by bilirubin.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第2期293-296,共4页
Chinese Journal of Pathophysiology
